Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the anti hypertensive effect of hydrolysate in spontaneously hypertensive rats
文献类型：期刊论文
| 作者 | Wang, Jiapei1,2; Hu, Jianen3; Cui, Jinzhe4; Bai, Xuefang1; Du, Yuguang1; Miyaguchi, Yuji5; Lin, Bingcheng1 |
| 刊名 | food chemistry
 |
| 出版日期 | 2008-11-15 |
| 卷号 | 111期号:2页码:302-308 |
| 关键词 | oyster protein hydrolysate ACE inhibitory peptides spontaneously hypertensive rat purification and identification hypertension |
| 英文摘要 | oyster (crassostrea talienwhanensis crosse) proteins were produced from fresh oyster and subsequently digested with pepsin. the separations were performed with a sephadex lh-20 gel filtration chromatography and a rp-hplc. a purified peptide with sequence val-val-tyr-pro-trp-thr-gln-arg-phe (vvypwtqrf) was firstly isolated and characterized from oyster protein hydrolysate and its ace inhibitory activity was determined with ic50 value of 66 mu mol/l in vitro. stability study for ace inhibitory activity showed that the isolated nonapeptide had the good heat and ph stability and strong enzyme-resistant properties against gastrointestinal proteases. kinetic experiments demonstrated that inhibitory kinetic mechanism of this peptide was non-competitive and its k-m and k-i values were calculated. the yield of this peptide from oyster proteins was 8.5%. furthermore, the oyster protein hydrolysate (fraction ii), prepared by pepsin treatment firstly exhibited antihypertensive activity when it was orally administered to spontaneously hypertensive rat (shr) at a dose of 20 mg/kg. these results demonstrated that the hydrolysate from oyster proteins prepared by pepsin treatment could serve as a source of peptides with antihypertensive activity. (c) 2008 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, applied ; food science & technology ; nutrition & dietetics |
| 研究领域[WOS] | chemistry ; food science & technology ; nutrition & dietetics |
| 关键词[WOS] | angiotensin-converting enzyme ; antihypertensive peptides ; endogenous inhibitor ; bovine brain ; vv-hemorphin-7 ; hemoglobin ; hemorphins ; sauce ; assay ; milk |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000257979700005 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141038]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grp 1805, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Dalian Fisheries Univ, Dalian 116023, Peoples R China 4.Dalian Inst Light Ind, Sch Art & Design, Dalian 116034, Peoples R China 5.Ibaraki Univ, Coll Agr, Ibaraki 3000393, Japan |
| 推荐引用方式 GB/T 7714 | Wang, Jiapei,Hu, Jianen,Cui, Jinzhe,et al. Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the anti hypertensive effect of hydrolysate in spontaneously hypertensive rats[J]. food chemistry,2008,111(2):302-308. |
| APA | Wang, Jiapei.,Hu, Jianen.,Cui, Jinzhe.,Bai, Xuefang.,Du, Yuguang.,...&Lin, Bingcheng.(2008).Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the anti hypertensive effect of hydrolysate in spontaneously hypertensive rats.food chemistry,111(2),302-308. |
| MLA | Wang, Jiapei,et al."Purification and identification of a ACE inhibitory peptide from oyster proteins hydrolysate and the anti hypertensive effect of hydrolysate in spontaneously hypertensive rats".food chemistry 111.2(2008):302-308. |
入库方式： OAI收割
来源：大连化学物理研究所
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