Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples
文献类型：期刊论文
| 作者 | Wang, Yuan1; Lehmann, Rainer2; Lu, Xin1; Zhao, Xinjie1; Xu, Guowang1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2008-09-12 |
| 卷号 | 1204期号:1页码:28-34 |
| 关键词 | HPLC column-switching HILIC hydrophobic hydrophilic metabonomics |
| 英文摘要 | in this study we developed and optimized a column-switching high-performance liquid chromatographic (hplc) system for the complementary analysis of polar and apolar compounds in complex samples. the polar compounds are separated on a hydrophilic interaction chromatographic (hilic) column; the part of the sample non-retained on hilic is transferred into and separated on a reversed-phase (rp) column through an interface, thus preventing the loss of analytes eluting at dead time in common single-column mode. the signals are in turn recorded in the same chromatogram either by a uv or by a mass spectrometric detector. applying a mixture of 25 standard compounds (12 hydrophilic and 13 hydrophobic) the system proves to be reliable, robust and easy-operating. the investigation of urine revealed an increase in the detected metabolite ion masses compared to the single-column hilic-electrospray ionization (esi)-ms analysis. utilizing online the complementary selectivity of hilic and rplc in a fully automatic mode, this approach holds out the prospect to enlarge the number of detectable compounds in non-targeted "-omics" studies and sophisticated target-driven approaches by a single injection. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; comprehensive 2-dimensional separations ; reversed-phase ; fluorescence detection ; microdialysis samples ; peak-capacity ; human plasma ; ms method ; esi-ms ; hplc |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259516400004 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141071]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Univ Hosp Tuebingen, Dept Internal Med 4, Cent Lab, Div Clin Chem & Pathobiochem, D-72076 Tubingen, Germany |
| 推荐引用方式 GB/T 7714 | Wang, Yuan,Lehmann, Rainer,Lu, Xin,et al. Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples[J]. journal of chromatography a,2008,1204(1):28-34. |
| APA | Wang, Yuan,Lehmann, Rainer,Lu, Xin,Zhao, Xinjie,&Xu, Guowang.(2008).Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples.journal of chromatography a,1204(1),28-34. |
| MLA | Wang, Yuan,et al."Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples".journal of chromatography a 1204.1(2008):28-34. |
入库方式： OAI收割
来源：大连化学物理研究所
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