Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis
文献类型：期刊论文
| 作者 | Xu, Weijia2; Gao, Peng1; Zhang, Xiaohui2; Song, Liguo3 |
| 刊名 | new microbiologica
 |
| 出版日期 | 2008-07-01 |
| 卷号 | 31期号:3页码:377-381 |
| 关键词 | Ceftazidime capillary electrophoresis quality control |
| 英文摘要 | good quality of antibiotic discs is a fundamental prerequisite to accurate antibiotic susceptibility tests. capillary electrophoresis (ce) is a widely used method for quantitative analysis. here, using ceftazidime as an example, we report an easy-to-perform strategy to determine ceftazidime content in discs. first, a serial of ceftazidime standard solutions was prepared to determine the detection linearity. utilizing the background buffer containing 50 mmol/l na2hpo4, 0.045 mmol/l p-cyclodextrin and 3.15 mmol/l tris (hydroxymethyl) aminomethane, ceftazidime of concentrations ranging from 1.875 to 60 mu g/ml showed a linear response to detected peak areas. subsequently, four kinds of ceftazidime discs were selected from different manufacturers. the discs were homogenized using 1 ml deionized water, and then detected by ce after filtration. the results showed that one kind of discs were of poor quality as further confirmed by disc diffusion tests using standard strains. this study proved the potential of ce as an easy choice to perform disc quality control under appropriate conditions. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | microbiology |
| 研究领域[WOS] | microbiology |
| 关键词[WOS] | staphylococcus-aureus ; quality-control ; resistance ; susceptibility |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259559800011 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141076]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Dalian Municipal Cent Hosp, Dalian 116021, Peoples R China 3.Dalian Econ & Technol Dev Zone, Ctr Dis Control, Dalian 116000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Weijia,Gao, Peng,Zhang, Xiaohui,et al. Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis[J]. new microbiologica,2008,31(3):377-381. |
| APA | Xu, Weijia,Gao, Peng,Zhang, Xiaohui,&Song, Liguo.(2008).Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis.new microbiologica,31(3),377-381. |
| MLA | Xu, Weijia,et al."Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis".new microbiologica 31.3(2008):377-381. |
入库方式： OAI收割
来源：大连化学物理研究所
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