Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation
文献类型：期刊论文
| 作者 | Chen, Zhiyong1; Xu, Li2; Liang, Yuan1; Wang, Jianbin1; Zhao, Meiping1; Li, Yuanzong1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2008-02-22 |
| 卷号 | 1182期号:1页码:128-131 |
| 关键词 | HPLC monolith cryogel nanoparticle protein |
| 英文摘要 | a supermacroporous monolithic cryogel was directly prepared by in situ cryo-copolymerization in a stainless steel cartridge (70 mm x 5.0 mm i.d.) using methacrylic acid (maa) as functional monomer;and polyethylene glycol diacrylate (pegda) as crosslinker. the highly crosslinked (90%, molar ratio) poly(maa-pegda) cryogel had more uniform supermacropores with a mean diameter of 25 p,m compared to the poly(acrylamide)-based cryogels. the viability of poly(maa-pegda) cryogel as a medium was demonstrated for separations of lysozyme from chicken egg white (cew) and water-soluble poly(n-isopropylacrylamide-co-3-(dimethylamino) propyl methacrylamide) (nipam-dmapma) nanoparticles from its crude reaction solution. the dynamic binding capacities of lysozyme and the polymeric nanoparticles were 4.51 x 10(-3) mu mol/ml and 33.4 mu g/ml, respectively. the lysozyme recovered from the above separations had a purity of more than 85%, and retained 90% of its enzymatic activity. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | affinity ; purification ; columns |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253869800013 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141079]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, BNLMS, Beijing 100871, Peoples R China 2.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhiyong,Xu, Li,Liang, Yuan,et al. Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation[J]. journal of chromatography a,2008,1182(1):128-131. |
| APA | Chen, Zhiyong,Xu, Li,Liang, Yuan,Wang, Jianbin,Zhao, Meiping,&Li, Yuanzong.(2008).Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation.journal of chromatography a,1182(1),128-131. |
| MLA | Chen, Zhiyong,et al."Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation".journal of chromatography a 1182.1(2008):128-131. |
入库方式： OAI收割
来源：大连化学物理研究所
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