Analysis of acetylcholine, choline and butyrobetaine in human liver tissues by hydrophilic interaction liquid chromatography-tandem mass spectrometry
文献类型：期刊论文
| 作者 | Wang, Yuan2; Wang, Tao1; Shi, Xianzhe2; Wan, Dafang1; Zhang, Pingping1; He, Xianghuo1; Gao, Peng2; Yang, Shengli1,2,3; Gu, Jianren1; Xu, Guowang2 |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2008-08-05 |
| 卷号 | 47期号:4-5页码:870-875 |
| 关键词 | HILIC tandem mass spectrometry acetylcholine choline butyrobetaine tissue liver |
| 英文摘要 | the strong polar quaternary ammoniums, acetylcholine (ach), choline (ch) and butyrobetaine (bb, (3-carboxypropyl)trimethyl ammonium), are believed playing important roles in liver metabolism. these metabolites are at low levels and are weakly retained on reversed-phase liquid chromatographic (rp-lc) columns. several hydrophilic interaction liquid chromatography-tandem mass spectrometry (hilic-ms/ms) methods have been reported to analyze these compounds from different samples. however, no application to human liver tissues has been published. in this study, hilic-ms/ms method was developed to simultaneously determine these three metabolites in human liver tissues. they were simply extracted from tissue, separated on a hilic column, and detected by tandem ms in the mode of multiple reaction monitoring (mrm). further studies on the recovery and repeatability based on real samples indicated the method was accurate and reliable. this method was successfully applied to measure the levels of ach, ch and bb in 61 human liver tissue samples including normal, hepatocellular carcinoma (hcc) and matched non-cancerous liver tissues. by comparison of ch and ach contents in 29 hcc with their matched non-cancerous liver tissues, it was found that ach content increased in 11/29 hcc cases and decreased in 13/29 cases. furthermore, the ach/ch ratio increased in 16/29 hcc cases, while it decreased in 8/29 cases. these results strongly indicated that there exist different patterns of ach content in cancer tissues among hcc patients, thus highlighting the understanding of ach and its relevant signal pathways in hepatic carcinogenesis and hcc progression. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | small polar compounds ; microdialysis samples ; basal acetylcholine ; peptide separation ; enzyme reactors ; silica columns ; rat-brain ; plasma ; phase ; acid |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000257220300033 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141123]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Canc Inst, State Key Lab Oncogenes & Related Genes, Shanghai 200032, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yuan,Wang, Tao,Shi, Xianzhe,et al. Analysis of acetylcholine, choline and butyrobetaine in human liver tissues by hydrophilic interaction liquid chromatography-tandem mass spectrometry[J]. journal of pharmaceutical and biomedical analysis,2008,47(4-5):870-875. |
| APA | Wang, Yuan.,Wang, Tao.,Shi, Xianzhe.,Wan, Dafang.,Zhang, Pingping.,...&Xu, Guowang.(2008).Analysis of acetylcholine, choline and butyrobetaine in human liver tissues by hydrophilic interaction liquid chromatography-tandem mass spectrometry.journal of pharmaceutical and biomedical analysis,47(4-5),870-875. |
| MLA | Wang, Yuan,et al."Analysis of acetylcholine, choline and butyrobetaine in human liver tissues by hydrophilic interaction liquid chromatography-tandem mass spectrometry".journal of pharmaceutical and biomedical analysis 47.4-5(2008):870-875. |
入库方式： OAI收割
来源：大连化学物理研究所
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