Resurveying the Tris buffer solution: The specific interaction between tris(hydroxymethyl)aminomethane and lysozyme
文献类型：期刊论文
| 作者 | Quan, Li; Wei, Dengguo; Jiang, Xiaolu; Liu, Yang; Li, Zhiyu; Li, Na; Li, Kean; Liu, Feng; Lai, Luhua |
| 刊名 | analytical biochemistry
 |
| 出版日期 | 2008-07-15 |
| 卷号 | 378期号:2页码:144-150 |
| 关键词 | lysozyme tris(hydroxymethyl)aminomethane protein-buffer binding quartz crystal microbalance biosensor surface plasmon resonance molecular docking |
| 英文摘要 | an unusual phenomenon, the specific interaction between tris(hydroxymethyl)aminomethane (tris) and lysozyme (lzm), was demonstrated for the first time by rapid screen analysis of interactions using a quartz crystal microbalance (qcm) biosensor. this phenomenon was also observed in a surface plasmon resonance (spr) system. further study using high-performance affinity chromatography (hpac) confirmed this specific interaction between lzm and immobilized tris with an apparent dissociation constant (k-d) of 6.7 x 10(-5) m. molecular docking was carried out to identify possible modes of binding between lzm and tris linked to a binding arm. the estimated binding free energy was -6.34 kcal mol(-1), corresponding to a k-d of 2.3 x 10(-5) m, which correlated well with the experimental value. based on the docking model, the three hydroxyl groups of tris form intermolecular h bonds with asp52, glu35, and ala107 in lzm. this study reinforces the importance of buffer selection in quantitative biochemical investigations. for a lysozyme ligand binding study, it is better to avoid using tris when the ligands under study are weak binders. (c) 2008 elsevier inc. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | quartz-crystal microbalance ; affinity-chromatography ; binding ; recognition ; biosensor ; ligands ; protein ; surface |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000256846800006 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141205]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Quan, Li,Wei, Dengguo,Jiang, Xiaolu,et al. Resurveying the Tris buffer solution: The specific interaction between tris(hydroxymethyl)aminomethane and lysozyme[J]. analytical biochemistry,2008,378(2):144-150. |
| APA | Quan, Li.,Wei, Dengguo.,Jiang, Xiaolu.,Liu, Yang.,Li, Zhiyu.,...&Lai, Luhua.(2008).Resurveying the Tris buffer solution: The specific interaction between tris(hydroxymethyl)aminomethane and lysozyme.analytical biochemistry,378(2),144-150. |
| MLA | Quan, Li,et al."Resurveying the Tris buffer solution: The specific interaction between tris(hydroxymethyl)aminomethane and lysozyme".analytical biochemistry 378.2(2008):144-150. |
入库方式： OAI收割
来源：大连化学物理研究所
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