Comprehensive two-dimensional liquid chromatography (NPLC x RPLC) with vacuum-evaporation interface
文献类型：期刊论文
| 作者 | Tian, Hongzhe1,2; Xu, Jing1; Guan, Yafeng1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 31期号:10页码:1677-1685 |
| 关键词 | comprehensive two-dimensional liquid chromatography NPLC x RPLC radix salviae miltiorrhiza bage traditional Chinese medicines vacuum-evaporation interface |
| 英文摘要 | a comprehensive two-dimensional liquid chromatographic system incorporating a vacuum-evaporation interface was developed. normal-phase liquid chromatography with a cn microcolumn was used as the first dimension (1(st)-d), and reversed-phase liquid chromatography with a c-18 monolithic column was used as the second dimension (2(nd) -d). an electronically controlled dual-position, ten-port valve with two identical storage loops served as the interface and the analysis time in the 2(nd)-d was 1.5 min. the solvent in the loops of the interface was evaporated at 25 degrees c under vacuum conditions, leaving the analytes on the inner wall of the loops. the mobile phase of the 2(nd) -d dissolved the analytes in the loop and injected them onto the second column, allowing an on-line solvent exchange of the fractions from the 1(st)-d to the 2(nd)-d. the chromatographic resolution of analytes on the two dimensions was evaluated. sample loss due to evaporation in the interface was investigated with standard samples having different boiling points. the usefulness of the comprehensive 2-dlc system was demonstrated in the analysis of a traditional chinese medicine radix salviae miltiorrhiza bage extract. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | separation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000256987500004 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141235]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China 2.Shenyang Agr Univ, Analyt Ctr, Shenyang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tian, Hongzhe,Xu, Jing,Guan, Yafeng. Comprehensive two-dimensional liquid chromatography (NPLC x RPLC) with vacuum-evaporation interface[J]. journal of separation science,2008,31(10):1677-1685. |
| APA | Tian, Hongzhe,Xu, Jing,&Guan, Yafeng.(2008).Comprehensive two-dimensional liquid chromatography (NPLC x RPLC) with vacuum-evaporation interface.journal of separation science,31(10),1677-1685. |
| MLA | Tian, Hongzhe,et al."Comprehensive two-dimensional liquid chromatography (NPLC x RPLC) with vacuum-evaporation interface".journal of separation science 31.10(2008):1677-1685. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。