Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA
文献类型：期刊论文
| 作者 | Lv Wei1,3; Chen XuDong1,3; Aumiler, Damir1,2,3; Xia AnDong1,3 |
| 刊名 | science in china series b-chemistry
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 52期号:8页码:1148-1153 |
| 关键词 | cyanine dyes single molecule spectroscopy on/off blinking fluorescence correlation spectroscopy (FCS) |
| 英文摘要 | the intersystem crossing and isomerization dynamics of free-cy3, cy3-ssdna, free-cy5 and cy5-ssdna are obtained through simple analysis of rapid on/off blinking from single molecule fluorescence intensity time-traces and the fluorescence correlation spectroscopy (fcs). the on- and off-times observed in fluorescence time traces of single cyanine dyes are due to the formation of the triplet state and isomerization, where both the interaction with dna and long central polymethine chain of cyanine dyes increase the barriers of isomerization, leading to long off-time. the results indicate that the single molecule fluorescence fluctuation together with the resulting second autocorrelation analysis are powerful methods for determining the triplet state and isomerization dynamics, which could be the simple techniques and complementary to other spectroscopic techniques, such as fluorescence decay measurement and laser flash photolysis to study the photophysical processes of complex molecules. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | photoinduced isomerization ; back-isomerization ; room-temperature ; glass-surface ; cy5 ; spectroscopy ; intensity ; photophysics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268514800012 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141291]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing 100190, Peoples R China 2.Inst Phys, Zagreb 10000, Croatia 3.Chinese Acad Sci, Inst Chem, BNLMS, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv Wei,Chen XuDong,Aumiler, Damir,et al. Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA[J]. science in china series b-chemistry,2009,52(8):1148-1153. |
| APA | Lv Wei,Chen XuDong,Aumiler, Damir,&Xia AnDong.(2009).Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA.science in china series b-chemistry,52(8),1148-1153. |
| MLA | Lv Wei,et al."Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA".science in china series b-chemistry 52.8(2009):1148-1153. |
入库方式： OAI收割
来源：大连化学物理研究所
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