Novel surface modified molecularly imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers for selective recognition of lysozyme in aqueous solution
文献类型：期刊论文
| 作者 | Zhang, Wei1; Qin, Lei1; He, Xi-Wen1; Li, Wen-You1; Zhang, Yu-Kui1,2 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2009-05-22 |
| 卷号 | 1216期号:21页码:4560-4567 |
| 关键词 | Surface molecular imprinting Silica beads beta-Cyclodextrin Lysozyme High-performance liquid chromatography Protein separation |
| 英文摘要 | a novel protein imprinted polymer for selective recognition of lysozyme was obtained. acryloy-beta-cyclodextrin, which offered hydrophilic exterior and hydrophobic cavity that were allowed to self-assemble with the template protein through hydrogen interaction and hydrophobic interaction, was synthesized and used as the functional monomer. polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a new type of protein imprinted polymer. langmuir adsorption model was employed to describe the isotherms. and maximum adsorption capacity was evaluated. the performance of such imprinted polymer was further demonstrated by high-performance liquid chromatography, and the results showed that the column packed with the lysozyme imprinted silica beads could effectively separate lysozyme from the mixture of lysozyme-cytochrome c, lysozyme-bovine serum albumin, lysozyme-avidin or lysozyme-methylated bovine serum albumin, which showed its high selectivity. (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | protein recognition ; antibodies ; tryptophan ; separation ; peptides ; media ; beads ; water ; ion ; gel |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266175100004 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141300]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wei,Qin, Lei,He, Xi-Wen,et al. Novel surface modified molecularly imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers for selective recognition of lysozyme in aqueous solution[J]. journal of chromatography a,2009,1216(21):4560-4567. |
| APA | Zhang, Wei,Qin, Lei,He, Xi-Wen,Li, Wen-You,&Zhang, Yu-Kui.(2009).Novel surface modified molecularly imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers for selective recognition of lysozyme in aqueous solution.journal of chromatography a,1216(21),4560-4567. |
| MLA | Zhang, Wei,et al."Novel surface modified molecularly imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers for selective recognition of lysozyme in aqueous solution".journal of chromatography a 1216.21(2009):4560-4567. |
入库方式： OAI收割
来源：大连化学物理研究所
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