Simultaneous derivatization and extraction of free cyanide in biological samples with home-made hollow fiber-protected headspace liquid-phase microextraction followed by capillary electrophoresis with UV detection
文献类型：期刊论文
| 作者 | Meng, Liang1; Liu, Xin2; Wang, Bin2; Shen, Guijun1; Wang, Zhengqiao2; Guo, Ming1,3 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2009-11-01 |
| 卷号 | 877期号:29页码:3645-3651 |
| 关键词 | Headspace liquid-phase microextraction Home-made hollow fiber membrane Free cyanide Capillary electrophoresis Urine Saliva |
| 英文摘要 | a new method is reported for the simultaneous derivatization and extraction of free cyanide in biological samples using home-made hollow fiber-protected headspace liquid-phase microextraction (hf-hs-lpme) followed by capillary electrophoresis (ce) determination. the acceptor phase containing ni(ii)-nh(3) (as derivatization agent), sodium carbonate and ammonium pyromellitate (as internal standard) is held within a hollow fiber membrane, affixed to a syringe needle and immersed in the headspace of sample container. the extracted cyanide from the neutral samples forms a stable ni(cn)(4)(2-) complex which is determined by ce. parameters affecting extraction efficiency were investigated and optimized. for optimum peak shapes, the capillary was coated with cetyltrimethylammonium bromide (ctab). the calibration curve was linear for concentrations of cn(-) in the range from 0.1 to 20 mu mol l(-1) (r(2) = 0.9987). the lod (s/n = 3) was estimated to be 0.01 mu mol l(-1) of cn(-) concentration. such a detection sensitivity is high enough for free cyanide determination in common environmental and biological samples. excellent repeatability of the extraction (rsd <= 5.6%, n = 5) was achieved. the feasibility of this method was demonstrated by analyzing human urine and saliva samples with spiked recoveries in the range of 92-103.4%. this work provided an efficient alternative to the present headspace microextraction techniques such as headspace solid-phase microextraction (hs-spme) and headspace single-drop microextraction (hs-sdme). (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | spectrophotometric determination ; fluorescence detection ; single-drop ; water ; ion ; chromatography ; blood |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000271168100024 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141355]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ Technol, Coll Environm & Chem Engn, Dalian 116622, Peoples R China 2.Dalian Publ Secur Bur, Forens Sci Inst, Dalian 116030, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Liang,Liu, Xin,Wang, Bin,et al. Simultaneous derivatization and extraction of free cyanide in biological samples with home-made hollow fiber-protected headspace liquid-phase microextraction followed by capillary electrophoresis with UV detection[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2009,877(29):3645-3651. |
| APA | Meng, Liang,Liu, Xin,Wang, Bin,Shen, Guijun,Wang, Zhengqiao,&Guo, Ming.(2009).Simultaneous derivatization and extraction of free cyanide in biological samples with home-made hollow fiber-protected headspace liquid-phase microextraction followed by capillary electrophoresis with UV detection.journal of chromatography b-analytical technologies in the biomedical and life sciences,877(29),3645-3651. |
| MLA | Meng, Liang,et al."Simultaneous derivatization and extraction of free cyanide in biological samples with home-made hollow fiber-protected headspace liquid-phase microextraction followed by capillary electrophoresis with UV detection".journal of chromatography b-analytical technologies in the biomedical and life sciences 877.29(2009):3645-3651. |
入库方式： OAI收割
来源：大连化学物理研究所
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