Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry for metabonomics: Biomarker discovery for diabetes mellitus
文献类型：期刊论文
| 作者 | Li, Xiang1; Xu, Zhiliang; Lu, Xin1; Yang, Xuehui2; Yin, Peiyuan1; Kong, Hongwei1; Yu, Ying3; Xu, Guowang1 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2009-02-09 |
| 卷号 | 633期号:2页码:257-262 |
| 关键词 | Metabonomics Metabolomics GC x GC-TOFMS Biomarker Diabetes mellitus |
| 英文摘要 | comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (gc x gc-tofms) coupled with pattern recognition methods was applied to analyze plasma from diabetic patients and healthy controls. after sample preparation and gc x gc-tcfms analysis, collected data were transformed, the peak alignment between different chromatograms was performed to generate the metabolites' peak table, then orthogonal signal correction filtered partial least-squares discriminant analysis (osc-plsda) was carried out to model the data and discover metabolites with a significant concentration change in diabetic patients. with the method above, diabetic patients and healthy controls could be correctly distinguished based on the metabolic abnormity in plasma. five potential biomarkers including glucose, 2-hydroxyisobutyric acid, linoleic acid, palmitic acid and phosphate were identified. it was found that elevated free fatty acids were essential pathophysiological factors in diabetes mellitus which reflected either the hyperglycemia or the deregulation of fatty acids metabolism. these potential biomarkers in plasma, e.g. palmitic acid, linoleic acid and 2-hydroxybutyric acid might be helpful in the diagnosis or further study of diabetes mellitus. this study shows the practicability and advantage of gc x gc-tofms coupled with data analysis and mining for metabonomics in biomarker discovery. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | x gc-tofms ; fatty-acid cycle ; insulin-resistance ; tissue-extracts ; metabolites ; plasma ; metabolomics ; deconvolution ; performance ; platform |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263188400014 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141388]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Zhejiang Univ Tradit Chinese Med, Affiliated Hosp 1, Res Ctr Clin Lab, Hangzhou 310006, Zhejiang, Peoples R China 3.Zhejiang Tradit Chinese Med Univ, Affiliated Hosp 1, Dept Endocrinol, Hangzhou 310006, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiang,Xu, Zhiliang,Lu, Xin,et al. Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry for metabonomics: Biomarker discovery for diabetes mellitus[J]. analytica chimica acta,2009,633(2):257-262. |
| APA | Li, Xiang.,Xu, Zhiliang.,Lu, Xin.,Yang, Xuehui.,Yin, Peiyuan.,...&Xu, Guowang.(2009).Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry for metabonomics: Biomarker discovery for diabetes mellitus.analytica chimica acta,633(2),257-262. |
| MLA | Li, Xiang,et al."Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry for metabonomics: Biomarker discovery for diabetes mellitus".analytica chimica acta 633.2(2009):257-262. |
入库方式： OAI收割
来源：大连化学物理研究所
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