Microchip free flow isoelectric focusing for protein prefractionation using monolith with immobilized pH gradient
文献类型：期刊论文
| 作者 | Han, Bin1,2; Wang, Pingli1; Zhu, Guijie1; Zhang, Lihua1; Qu, Feng2; Deng, Yulin2; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 32期号:8页码:1211-1215 |
| 关键词 | Free flow electrophoresis IEF IPG Microchip Monolithic materials |
| 英文摘要 | microchip free flow ief (mu ffief) with monolithic ipg was proposed for protein prefractionation. the monolithic materials were first prepared by uv irradiation in a microchamber of 4:3 mm length, 23 mm width, and 38 mu m depth. carrier ampholytes (cas) were further immobilized on the monolith by chemical bonding, to form a stable ph gradient. by such a technique, the continuous introduction of cas ill traditional mu ffief could be avoided, not only to decrease the operation cost, but also to avoid the interference of cas for the further protein identification by ms/ms. with a fluorescence microscope as the detector, under the optimal conditions, the separation of fitc labeled beta-lactoglobulin and carbonic anhydrase. with 0.9 unit difference on pl, was achieved with good reproducibility. the experimental results demonstrated that under the experimental conditions we applied, the separation mechanism of mu ffief with m-tpg materials might be the cooperative effects of ief and cze, and the former one plays a predominant role. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | electrophoresis ; device ; resolution ; column ; speed ; chip |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265793700012 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141433]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Bin,Wang, Pingli,Zhu, Guijie,et al. Microchip free flow isoelectric focusing for protein prefractionation using monolith with immobilized pH gradient[J]. journal of separation science,2009,32(8):1211-1215. |
| APA | Han, Bin.,Wang, Pingli.,Zhu, Guijie.,Zhang, Lihua.,Qu, Feng.,...&Zhang, Yukui.(2009).Microchip free flow isoelectric focusing for protein prefractionation using monolith with immobilized pH gradient.journal of separation science,32(8),1211-1215. |
| MLA | Han, Bin,et al."Microchip free flow isoelectric focusing for protein prefractionation using monolith with immobilized pH gradient".journal of separation science 32.8(2009):1211-1215. |
入库方式： OAI收割
来源：大连化学物理研究所
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