Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes
文献类型：期刊论文
| 作者 | Guo, Weihua2; Ye, Zhiqiang1; Wang, Guilan1; Zhao, Xiaoming2; Yuan, Jingli1; Du, Yuguang2 |
| 刊名 | talanta
 |
| 出版日期 | 2009-05-15 |
| 卷号 | 78期号:3页码:977-982 |
| 关键词 | Oligochitosan Tobacco cells Interaction Europium complex Time-resolved fluorescence |
| 英文摘要 | the interaction of oligochitosan and tobacco cells has been investigated by fluorometric method using two eu(3+) complexes as the probes in this work. based on the reaction of tobacco cells with oligochitosan conjugated to a strongly fluorescent eu(3+) complex 4,4'-bis(1 '',1 '',1 '',2 '',2 '',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl)chlorosulfo-o-terphenyl-eu(3+) (oligochitosan-bhhct-eu(3+) conjugate), the binding kinetic process of oligochitosan-tobacco cells was fluorescently imaged. the results indicate that oligochitosan can be specifically bound to the walls as well as the membranes of tobacco cells. a sensitive and selective eu(3+) complex luminescence probe specific for singlet oxygen, [4'-(10-methyl-9-anthryl)-2,2':6',2 ''-terpyridine-6,6 ''-diyl]bis(methylenenitrilo)tetrakis (acetate)-eu(3+), was used for developing a new time-resolved fluorescence assay method for the determinations of indole-3-acetic acid (iaa) and peroxidase produced in the cells during the interaction of oligochitosan and tobacco cells. the assays are sensitive with the detection limits of 32 nm for iaa, and 1.2 nm for peroxidase, respectively. the concentration changes of iaa and peroxidase induced by oligochitosan in tobacco cells reveal that oligochitosan can effectively induce the increase of iaa concentration, accompanied by the decrease of peroxidase concentration. these results give a primary and reliable evidence to explain the growth-promoting mechanism of oligochitosan on the plants at molecular level. (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | time-resolved fluoroimmunoassay ; catalyzed aerobic oxidation ; singlet oxygen production ; energy-transfer ; in-vitro ; resistance ; acid ; chitooligosaccharides ; chitosan ; enzyme |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264976600055 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141466]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116012, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Weihua,Ye, Zhiqiang,Wang, Guilan,et al. Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes[J]. talanta,2009,78(3):977-982. |
| APA | Guo, Weihua,Ye, Zhiqiang,Wang, Guilan,Zhao, Xiaoming,Yuan, Jingli,&Du, Yuguang.(2009).Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes.talanta,78(3),977-982. |
| MLA | Guo, Weihua,et al."Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes".talanta 78.3(2009):977-982. |
入库方式： OAI收割
来源：大连化学物理研究所
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