Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene
文献类型：期刊论文
| 作者 | Li, Huihui1,2; Liu, Yiquan1; Lin, Sen1; Yuan, Gu1 |
| 刊名 | chemistry-a european journal
 |
| 出版日期 | 2009 |
| 卷号 | 15期号:10页码:2445-2452 |
| 关键词 | DNA structures duplexes G-quadruplexes noncovalent interactions oncogenes |
| 英文摘要 | this study has demonstrated the formation of the g-quadruplex structure from the g-rich sequence in the promoter region of the bcl-2 oncogene; the formation could be induced by addition of nh(4)(+) or k(+) ions. the binding affinity and stoichiometry of seven small molecules with the g-quadruplex were examined by using f,si-ms. its well as cd and uv spectroscopy. the binding-affinity order was determined to be p1 approximate to p5 > p2 > p3 approximate to p4 > p7 > p6. in particular, the small-molecule induction of the structural transition between the g-quadruplex and duplex dna forms in this promoter region was investigated by esi-ms. we directly observed specific binding of dehydrocorydaline (p7) and cationic porphyrin (p5) in one system consisting of the g-quadruplex and the duplex dna, respectively. the results indicate that p7 selectively stabilizes the g-quadruplex and shifts the equalibrium toward g-quadruplex formation of the bcl-2 promoter, whereas p5 converts the g-quadruplex into the duplex dna, which results in strong and selective binding to the duplex form. therefore, ps and p7 with their attractive binding specificities could be considered as precursors for pathway-specific drug, design for regulation of bcl-2 oncogene transcription. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | ionization mass-spectrometry ; automated docking ; small-molecule ; dna ; binding ; proteins ; duplex ; distamycin ; transition ; expression |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264029500026 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141467]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Dept Chem Biol,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Nanjing Normal Univ, Jiangsu Key Lab Biofunct Mat, Sch Chem & Environm Sci, Nanjing 210097, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Huihui,Liu, Yiquan,Lin, Sen,et al. Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene[J]. chemistry-a european journal,2009,15(10):2445-2452. |
| APA | Li, Huihui,Liu, Yiquan,Lin, Sen,&Yuan, Gu.(2009).Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene.chemistry-a european journal,15(10),2445-2452. |
| MLA | Li, Huihui,et al."Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene".chemistry-a european journal 15.10(2009):2445-2452. |
入库方式： OAI收割
来源：大连化学物理研究所
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