Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications
文献类型：期刊论文
| 作者 | Zhang Yu1,2; Yu Hao1; Dong Xiu-Ling3; Qin Jian-Hua1; Lin Bin-Cheng1 |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2009-06-10 |
| 卷号 | 30期号:6页码:1128-1130 |
| 关键词 | Microfluidic chip Acrylamide hydrogel DNA probes immobilization Hybridization |
| 英文摘要 | a method of dna probes immobilization and specific target sequences capture in a microfluidic chip was presented. acrydite-modified dna probes are immobilized in silanized glass microfluidic channels via photopolymerization in a polyacrylamide matrix. the resulting polymeric, hydrogel plugs are porous under electrophoretic conditions, and the immobilized dna probes can be hybridized with fluorescence labeled complementary dna. the total analysis process can be completed within 5 min, and the limit of detection is 0.1 mu mol/l. this method is simple, rapid and feasible. the double-stranded dna can be chemically denatured, and the chip is reusable. the conditions for photopolymerization, hybridization, and denaturation were discussed as well. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | electrochemical biosensor ; hybridization ; assay ; oligonucleotide ; capture |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267873700014 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141489]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Dalian Third Municipal Hosp, Dalian 116033, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Yu,Yu Hao,Dong Xiu-Ling,et al. Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications[J]. chemical journal of chinese universities-chinese,2009,30(6):1128-1130. |
| APA | Zhang Yu,Yu Hao,Dong Xiu-Ling,Qin Jian-Hua,&Lin Bin-Cheng.(2009).Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications.chemical journal of chinese universities-chinese,30(6),1128-1130. |
| MLA | Zhang Yu,et al."Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications".chemical journal of chinese universities-chinese 30.6(2009):1128-1130. |
入库方式： OAI收割
来源：大连化学物理研究所
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