The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence
文献类型：期刊论文
| 作者 | Wei, Chunying1; Wang, Lihua1; Jia, Guoqing2; Zhou, Jun2; Han, Gaoyi1; Li, Can2 |
| 刊名 | biophysical chemistry
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 143期号:1-2页码:79-84 |
| 关键词 | Binding modes G-quadruplexes Interaction Porphyrin Spectroscopy |
| 英文摘要 | interactions of 5,10,15,20-tetrakis(n-methylpyridinium-4-yl)-21h,23h-porphyrin (tmpyp4) and 5,10,15,20-tetrakis(n-propylpyridinium-4-yl)-21h,23h-porphyrin (tprpyp4) with the parallel four-stranded (tg(4)t)4 g-quadruplex dna in 100 mm k+-containing buffer were studied using circular dichroism (cd) spectroscopy, visible absorption titration, and steady and time-resolved fluorescence spectroscopies. the results show that the binding stoichiometric ratios of both tmpyp4 and tprpyp4 to (tg(4)t)4 are 3:1. two types of independent and nonequivalent binding sites with the higher and lower binding affinities are confirmed, and the stronger and weaker binding constants are 9.44 x 10(7) and 6.94 x 10(5) m-1 for (tg(4)t)4-tmpyp4 complex, 7.86 x 10(7) and 6.35 x 10(5) m-1 for (tg(4)t)4-tprpyp4 complex, respectively. for both tmpyp4-(tg(4)t)4 and tprpyp4-(tg(4)t)4 complexes, one porphyrin molecule stacks on the one end of g-quadruplex with the higher binding affinity, another two porphyrins bind weakly to the two external grooves. the size of cation side arms around porphyrin core almost fails to affect the binding mode, stoichiometry and affinity of porphyrin to (tg(4)t)4 g-quadruplex in 100 mm k+-containing buffer. (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemistry & molecular biology ; biophysics ; chemistry, physical |
| 研究领域[WOS] | biochemistry & molecular biology ; biophysics ; chemistry |
| 关键词[WOS] | time-resolved fluorescence ; structural basis ; ligand-binding ; telomeric dna ; guanine ; complexes ; state ; stabilization ; selectivity ; tetraplex |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267006000009 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141535]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Shanxi Univ, Key Lab Chem Biol & Mol Engn, Minist Educ, Inst Mol Sci, Taiyuan 030006, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Chunying,Wang, Lihua,Jia, Guoqing,et al. The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence[J]. biophysical chemistry,2009,143(1-2):79-84. |
| APA | Wei, Chunying,Wang, Lihua,Jia, Guoqing,Zhou, Jun,Han, Gaoyi,&Li, Can.(2009).The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence.biophysical chemistry,143(1-2),79-84. |
| MLA | Wei, Chunying,et al."The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence".biophysical chemistry 143.1-2(2009):79-84. |
入库方式： OAI收割
来源：大连化学物理研究所
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