Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail
文献类型：期刊论文
| 作者 | Hu, Ying1,2; Luan, Hongwei1,2; Liu, Huixin1,2; Ge, Guangbo1,2; Zhou, Kun1,2; Liu, Yong1; Yang, Ling1 |
| 刊名 | bioscience biotechnology and biochemistry
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 73期号:3页码:671-676 |
| 关键词 | beta-D-glycosidase disaccharide synthesis broad acceptor specificity transfer efficiency China white jade snail |
| 英文摘要 | the acceptor specificity and transfer potential of a beta-d-glycosidase (g i), which had been purified from the china white jade snail, were further investigated by using various sugars as acceptors. g i had broad monosaccharide acceptor specificity for its transglycosylation activity. more specifically, it efficiently catalyzed the transfer of the beta-d-fucosyl, beta-d-glucosyl or beta-d-galactosyl moiety from the corresponding p-nitrophenyl-beta-d-glycopyranosides to various monosaccharides. the transfucosylation efficiency of g i was studied by using p-nitrophenyl-beta-d-fucopyranoside (pnpfuc) as the donor and glucose and xylose as the acceptors. the yields under conditions of non-initial velocity were 88% for glucose and 93% for xylose. the transfer product with glucose as the acceptor was isolated and identified as beta-fucosyl-1,6-glucose by an nmr analysis. the data from these analyses indicate that g i had broad acceptor specificity and high efficiency for transglycosylation. these uncommon properties of g i could make it a valuable biocatalyst for the synthesis of various disaccharides. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; chemistry, applied ; food science & technology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; chemistry ; food science & technology |
| 关键词[WOS] | bifidobacterium breve clb ; enzymatic-synthesis ; thermus-thermophilus ; aspergillus-oryzae ; escherichia-coli ; d-glucosidase ; transglycosylation ; oligosaccharides ; purification ; alpha |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264904900031 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141540]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Ying,Luan, Hongwei,Liu, Huixin,et al. Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail[J]. bioscience biotechnology and biochemistry,2009,73(3):671-676. |
| APA | Hu, Ying.,Luan, Hongwei.,Liu, Huixin.,Ge, Guangbo.,Zhou, Kun.,...&Yang, Ling.(2009).Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail.bioscience biotechnology and biochemistry,73(3),671-676. |
| MLA | Hu, Ying,et al."Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail".bioscience biotechnology and biochemistry 73.3(2009):671-676. |
入库方式： OAI收割
来源：大连化学物理研究所
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