A microfluidic DNA computing processor for gene expression analysis and gene drug synthesis
文献类型：期刊论文
| 作者 | Zhang, Yu1,2; Yu, Hao1; Qin, Jianhua1; Lin, Bingcheng1 |
| 刊名 | biomicrofluidics
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 3期号:4 |
| 关键词 | biochemistry biocomputing Boolean algebra cancer DNA genomics lab-on-a-chip microfluidics |
| 英文摘要 | boolean logic performs a logical operation on one or more logic input and produces a single logic output. here, we describe a microfluidic dna computing processor performing boolean logic operations for gene expression analysis and gene drug synthesis. multiple cancer-related genes were used as input molecules. their expression levels were identified by interacting with the computing related dna strands, which were designed according to the sequences of cancer-related genes and the suicide gene. when all the expressions of the cancer-related genes fit in with the diagnostic criteria, positive diagnosis would be confirmed and then a complete suicide gene (gene drug) could be synthesized as an output molecule. microfluidic chip was employed as an effective platform to realize the computing process by integrating multistep biochemical reactions involving hybridization, displacement, denaturalization, and ligation. by combining the specific design of the computing related molecules and the integrated functions of the microfluidics, the microfluidic dna computing processor is able to analyze the multiple gene expressions simultaneously and realize the corresponding gene drug synthesis with simplicity and fast speed, which demonstrates the potential of this platform for dna computing in biomedical applications. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biophysics ; nanoscience & nanotechnology ; physics, fluids & plasmas |
| 研究领域[WOS] | biochemistry & molecular biology ; biophysics ; science & technology - other topics ; physics |
| 关键词[WOS] | hydrogel plugs ; breast-cancer ; channels ; system ; chip ; polymorphism ; therapy ; machine ; assay |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273217500005 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141546]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yu,Yu, Hao,Qin, Jianhua,et al. A microfluidic DNA computing processor for gene expression analysis and gene drug synthesis[J]. biomicrofluidics,2009,3(4). |
| APA | Zhang, Yu,Yu, Hao,Qin, Jianhua,&Lin, Bingcheng.(2009).A microfluidic DNA computing processor for gene expression analysis and gene drug synthesis.biomicrofluidics,3(4). |
| MLA | Zhang, Yu,et al."A microfluidic DNA computing processor for gene expression analysis and gene drug synthesis".biomicrofluidics 3.4(2009). |
入库方式： OAI收割
来源：大连化学物理研究所
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