"Click dipeptide": A novel stationary phase applied in two-dimensional liquid chromatography
文献类型：期刊论文
| 作者 | Xue, Meiyun1; Huang, Hongxue1; Ke, Yanxiong1; Chu, Changhu1; Jin, Yu1; Liang, Xinmiao1,2 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2009-12-04 |
| 卷号 | 1216期号:49页码:8623-8629 |
| 关键词 | Click dipeptide stationary phase alpha-Azido L-phenylglycine dipeptide Orthogonality 2D-RP/RPLC Complex sample |
| 英文摘要 | 2d-hplc is an important technique for the separation of complex samples. developing new types of stationary phases is of great interest to construct 2d-lc systems with high orthogonality. in this study, a novel stationary phase-click dipeptide (l-phenylglycine dipeptide) was prepared by immobilization of alpha-azido l-phenylglycine dipeptide on alkyne-silica via click chemistry. in the preparation of this new material, an efficient, inexpensive and shelf-stable diazo transfer reagent (imidazole-1-sulfonyl azide hydrochloride) was utilized to transfer the amino group of l-phenylglycine to corresponding azido group under mild conditions. the click dipeptide thus prepared was confirmed by ft-ir, solid state cp/mas (13)c nmr and elemental analysis. the click dipeptide packing showed high orthogonality with c18, which reached 63.5%. an off-line 2d-rp/rplc system was developed to analyze a traditional chinese medicine (tcm)-rheum palmatum l. the results showed high orthogonality between click dipeptide and c18 as well as great separating power in the practical separation of complex samples. (c) 2009 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | isolated protein-fragments ; reversed-phase ; oligo(ethylene glycol) ; hplc system ; silica-gel ; separation ; chemistry ; peptides ; efficient ; columns |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272089200008 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141551]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Meiyun,Huang, Hongxue,Ke, Yanxiong,et al. "Click dipeptide": A novel stationary phase applied in two-dimensional liquid chromatography[J]. journal of chromatography a,2009,1216(49):8623-8629. |
| APA | Xue, Meiyun,Huang, Hongxue,Ke, Yanxiong,Chu, Changhu,Jin, Yu,&Liang, Xinmiao.(2009)."Click dipeptide": A novel stationary phase applied in two-dimensional liquid chromatography.journal of chromatography a,1216(49),8623-8629. |
| MLA | Xue, Meiyun,et al.""Click dipeptide": A novel stationary phase applied in two-dimensional liquid chromatography".journal of chromatography a 1216.49(2009):8623-8629. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。