Identification of Difference of Proteins Between Human Stomach Tumor and Healthy Tissues by Two-dimensional Liquid Chromatography-Mass Spectrometry
文献类型：期刊论文
| 作者 | Luo Fu-Wen2; Tao Ding-Yin1; Zhao Peng2; Zhang Li-Hua1; Jia Yu-Jie2; Zhang Yu-Kui1 |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 37期号:10页码:1403-1408 |
| 关键词 | Stomach cancer tissue proteomics two-dimensional high performance liquid chromatography-mass spectrometry |
| 英文摘要 | a two-dimensional high performance liquid chromatographic separation platform based on strong cation exchange (scx) chromatography and reversed phase ( rp) chromatography was developed. peptides were first separated by a scx column under stepwise gradient of 0, 20, 200,300, 500, 1000 and 2000 mmol/l nh4ac buffer. after each fraction was collected and desalted by a rp trap column, further separation was implemented on a rplc column under linear gradient elution. all the components were identified by mass spectrometry after 2d separation. the platform was applied to the analysis of proteins in stomach cancer tissue. the number of identified proteins were 537 and 506 for healthy and tumor tissue respectively. according to the physical characters of proteins identified, the distributions of these identified proteins were obtained and the difference between healthy and tumor tissue was found. the retrieval results were compared to screen out diagnostic proteins related to pathological changes of tumor tissues. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000271686300001 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141552]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Dept Gen Surg 2, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo Fu-Wen,Tao Ding-Yin,Zhao Peng,et al. Identification of Difference of Proteins Between Human Stomach Tumor and Healthy Tissues by Two-dimensional Liquid Chromatography-Mass Spectrometry[J]. chinese journal of analytical chemistry,2009,37(10):1403-1408. |
| APA | Luo Fu-Wen,Tao Ding-Yin,Zhao Peng,Zhang Li-Hua,Jia Yu-Jie,&Zhang Yu-Kui.(2009).Identification of Difference of Proteins Between Human Stomach Tumor and Healthy Tissues by Two-dimensional Liquid Chromatography-Mass Spectrometry.chinese journal of analytical chemistry,37(10),1403-1408. |
| MLA | Luo Fu-Wen,et al."Identification of Difference of Proteins Between Human Stomach Tumor and Healthy Tissues by Two-dimensional Liquid Chromatography-Mass Spectrometry".chinese journal of analytical chemistry 37.10(2009):1403-1408. |
入库方式： OAI收割
来源：大连化学物理研究所
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