Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail
文献类型：期刊论文
| 作者 | Hu, Ying1,2; Luan, Hongwei1,2; Ge, Guangbo1,2; Liu, Huixin1,2; Zhang, Yanyan1,2; Zhou, Kun1,2; Liu, Yong1; Yang, Ling1 |
| 刊名 | journal of biotechnology
 |
| 出版日期 | 2009-02-05 |
| 卷号 | 139期号:3页码:229-235 |
| 关键词 | beta-D-Glycosidase Transglycosylation activity Kinetics Allosteric effect The China white jade snail |
| 英文摘要 | a beta p-d-glycosidase (g1)from the china white jade snail showed non-michaelis-menten mode in catalyzing the reaction using pnpglu and pnpfuc as the substrate and monitoring the released pnp. we determined quantitatively both the transglycosidic and hydrolytic products of pnpglu and pnpfuc solvolysis for the detailed kinetic analysis on g1-catalyzed hydrolysis and transglycosylation reaction. the inhibition kinetic studies using deoxynojirimycin (dnj) and butanol as inhibitors were preceded. dnj only inhibited competitively the hydrolysis of cellobiose and pnpglu while "activated" the transglycosylation of pnpglu and pnpfuc. this was evident from the increased v(max)tr value with no change of the apparent k(m)tr. in contrast, butanol exhibited a competitive inhibition to the transglycosylation reaction and non-competitive inhibition to the hydrolysis. the results indicated that the non-michaelis-menten kinetic behavior was caused by the co-occurrence of substrate transglycosylation reaction. this study provided a simple method to increase the transglycosylation yield by using dnj to inhibit hydrolysis. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | thermostable beta-glycosidase ; d-glucosidase ; enzymatic characterization ; oligosaccharide synthesis ; thermotoga-neapolitana ; substrate-specificity ; thermus-thermophilus ; glycosyl hydrolases ; pyrococcus-furiosus ; aspergillus-niger |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263742300006 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141569]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Ying,Luan, Hongwei,Ge, Guangbo,et al. Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail[J]. journal of biotechnology,2009,139(3):229-235. |
| APA | Hu, Ying.,Luan, Hongwei.,Ge, Guangbo.,Liu, Huixin.,Zhang, Yanyan.,...&Yang, Ling.(2009).Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail.journal of biotechnology,139(3),229-235. |
| MLA | Hu, Ying,et al."Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail".journal of biotechnology 139.3(2009):229-235. |
入库方式： OAI收割
来源：大连化学物理研究所
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