Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel
文献类型：期刊论文
| 作者 | Li, Sheng-Hua; Wang, Jun; Zhao, Mei-Ping |
| 刊名 | journal of separation science
 |
| 出版日期 | 2009-10-01 |
| 卷号 | 32期号:19页码:3359-3363 |
| 关键词 | BSA-Cu(II) complex Coordinate interaction Molecularly imprinted hydrogel |
| 英文摘要 | a novel molecularly imprinted hydrogel for bovine serum albumin (bsa) was prepared using cupric ion as the bridge between the template bsa and the functional monomer 4-vinylpyridine. n-isopropylacrylamide (nipa) was used as an assistant monomer to provide the stimuli-responsibility of the polymer. the adsorption conditions of bsa on the bsa-cu(ii)-imprinted hydrogel were optimized considering the influences of ph, temperature, and salt concentration. the proteins bound on the imprinted hydrogel can be easily recovered under mild conditions by using 10 mmol/l ethylene diamine tetraacetic acid (edta) (ph 7.0) containing 150 mmol/l nacl as the eluting solution. the imprinting effect and adsorption capacity of the polymer were found to be significantly improved compared to the hydrogel prepared in the absence of cupric ion. the results demonstrated the advantages of using a template-metal ion-monomer coordination system to strengthen the interaction between the protein and monomer. the effects of different metals ions including zn(ii), ni(ii), co(ii), cd(ii), and al(iii) on the recognition ability of the bsa-cu(ii)-imprinted hydrogel were also investigated. the polymer showed high selectivity toward both the template protein and the cupric ion. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | electrostatic functional-groups ; polyacrylamide-gel beads ; protein recognition ; binding ; hemoglobin ; histidine ; polymers ; surfaces ; site |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270964800014 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141603]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Sheng-Hua,Wang, Jun,Zhao, Mei-Ping. Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel[J]. journal of separation science,2009,32(19):3359-3363. |
| APA | Li, Sheng-Hua,Wang, Jun,&Zhao, Mei-Ping.(2009).Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel.journal of separation science,32(19),3359-3363. |
| MLA | Li, Sheng-Hua,et al."Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel".journal of separation science 32.19(2009):3359-3363. |
入库方式： OAI收割
来源：大连化学物理研究所
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