Alkaloid profiling of the Chinese herbal medicine Fuzi by combination of matrix-assisted laser desorption ionization mass spectrometry with liquid chromatography-mass spectrometry
文献类型：期刊论文
| 作者 | Wang, Jiangshan1,2; van der Heijden, Rob1; Spijksma, Gerwin1; Reijmers, Theo1; Wang, Mei3; Xu, Guowang2; Hankemeier, Thomas1; van der Greef, Jan1,3 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2009-03-13 |
| 卷号 | 1216期号:11页码:2169-2178 |
| 关键词 | Alkaloid profiling Aconitum carmichaeli Debx Herbal medicine LC-MS MALDI-MS |
| 英文摘要 | a matrix-assisted laser desorption ionization mass spectrometry (maldi-ms) method was developed for the high throughput and robust qualitative profiling of alkaloids in fuzi-the processed lateral roots of the chinese herbal medicine aconitum carmichaeli debx (a. carmichaeli). after optimization, powdered roots - without any further sample preparation - could be used to screen for the presence or aconitum alkaloids. furthermore, the semi-quantitative potential of maldi-ms was confirmed using liquid chromatography-mass spectrometry (lc-ms) as reference. in total over sixty alkaloids were detected by lc-ms and fifteen of them were tentatively identified. both maldi-ms and lc-ms analysis revealed significant variation in alkaloid content in different (commercial) samples. lc-ms analysis of three toxic alkaloids in 14 batches of fuzi resulted in a variation of their concentrations expressed as rsds of 138%, 99% and 221% for aconitine, hypaconitine and mesaconitine, respectively. the variation in concentrations (expressed as rsd) of about the ninety constituents detected were classified as follows: 13 constituents showed an rsd of 77-100%, 46 with an rsd of 100-150%, 21 with an rsd of 150-200% and 9 constituents with an rsd in concentration of 200-235%. these results demonstrate a strong difference in chemical composition of the various fuzi and illustrate the necessity of adequate qa/qc procedures for both safety and efficiency of herbal medicine. the described analytical procedures for alkaloid profiling could play a role in these procedures. (c) 2008 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | aconitum alkaloids ; capillary-electrophoresis ; quantitative-determination ; urine samples ; body-fluids ; separation ; roots ; spectroscopy ; tubers ; tissue |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264226500026 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141613]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Leiden Univ, Leiden Amsterdam Ctr Drug Res, Div Analyt Biosci, NL-2300 RA Leiden, Netherlands 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.TNO, Qual Life & SU BioMed, NL-3700 AJ Zeist, Netherlands |
| 推荐引用方式 GB/T 7714 | Wang, Jiangshan,van der Heijden, Rob,Spijksma, Gerwin,et al. Alkaloid profiling of the Chinese herbal medicine Fuzi by combination of matrix-assisted laser desorption ionization mass spectrometry with liquid chromatography-mass spectrometry[J]. journal of chromatography a,2009,1216(11):2169-2178. |
| APA | Wang, Jiangshan.,van der Heijden, Rob.,Spijksma, Gerwin.,Reijmers, Theo.,Wang, Mei.,...&van der Greef, Jan.(2009).Alkaloid profiling of the Chinese herbal medicine Fuzi by combination of matrix-assisted laser desorption ionization mass spectrometry with liquid chromatography-mass spectrometry.journal of chromatography a,1216(11),2169-2178. |
| MLA | Wang, Jiangshan,et al."Alkaloid profiling of the Chinese herbal medicine Fuzi by combination of matrix-assisted laser desorption ionization mass spectrometry with liquid chromatography-mass spectrometry".journal of chromatography a 1216.11(2009):2169-2178. |
入库方式： OAI收割
来源：大连化学物理研究所
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