Urinary metabolic profiling of colorectal carcinoma based on online affinity solid phase extraction-high performance liquid chromatography and ultra performance liquid chromatography-mass spectrometry
文献类型：期刊论文
| 作者 | Wang, Wenzhao2; Feng, Bo1; Li, Xiang2; Yin, Peiyuan2; Gao, Peng2; Zhao, Xinjie2; Lu, Xin2; Zheng, Minhua1; Xu, Guowang2 |
| 刊名 | molecular biosystems
 |
| 出版日期 | 2010 |
| 卷号 | 6期号:10页码:1947-1955 |
| 英文摘要 | colorectal carcinoma (crc) is the third most commonly encountered cancer and fourth cause of cancer-associated death worldwide. abundant studies have demonstrated that one of the best effective therapies for enhancing the 5-year survival rate of patients is to diagnose the disease at an early stage. urine metabonomics is widely being utilized as an efficient platform to investigate the metabolic changes and discover the potential biomarkers of malignant diseases. in this study both ultra-performance liquid chromatography-mass spectrometry (uplc-ms) and online affinity solid phase extraction-high performance liquid chromatography (spe-hplc) were used to analyze the urinary metabolites from 34 healthy volunteers, 34 benign colorectal tumor and 50 colorectal carcinoma patients to produce comprehensive metabolic profiling data. a reliable separation between the control and disease groups as well as significantly changed metabolites were obtained from orthogonal signal correction partial least squares models which were built based on the two separate data sets from uplc-ms and affinity spe-hplc, respectively. 15 metabolites, showing the metabolic disorders of crc, were identified finally. these metabolites were found to be related to glutamine metabolism, fatty acid oxidation, nucleotide biosynthesis and protein metabolism. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | cancer-patients ; modified nucleosides ; tumor-markers ; fatty-acid ; metabonomics ; diagnosis ; therapy ; colon ; ms ; identification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281772300028 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141631]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Surg, Shanghai 200025, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Wenzhao,Feng, Bo,Li, Xiang,et al. Urinary metabolic profiling of colorectal carcinoma based on online affinity solid phase extraction-high performance liquid chromatography and ultra performance liquid chromatography-mass spectrometry[J]. molecular biosystems,2010,6(10):1947-1955. |
| APA | Wang, Wenzhao.,Feng, Bo.,Li, Xiang.,Yin, Peiyuan.,Gao, Peng.,...&Xu, Guowang.(2010).Urinary metabolic profiling of colorectal carcinoma based on online affinity solid phase extraction-high performance liquid chromatography and ultra performance liquid chromatography-mass spectrometry.molecular biosystems,6(10),1947-1955. |
| MLA | Wang, Wenzhao,et al."Urinary metabolic profiling of colorectal carcinoma based on online affinity solid phase extraction-high performance liquid chromatography and ultra performance liquid chromatography-mass spectrometry".molecular biosystems 6.10(2010):1947-1955. |
入库方式： OAI收割
来源：大连化学物理研究所
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