Zirconia layer coated mesoporous silica microspheres used for highly specific phosphopeptide enrichment
文献类型：期刊论文
| 作者 | Wan, Huihui; Yan, Jingyu; Yu, Long; Zhang, Xiuli; Xue, Xingya; Li, Xiuling; Liang, Xinmiao |
| 刊名 | talanta
 |
| 出版日期 | 2010-10-15 |
| 卷号 | 82期号:5页码:1701-1707 |
| 关键词 | Phosphopeptide enrichment Zirconia layer Mesoporous silica ESI-Q-TOF |
| 英文摘要 | zirconia layer coated mesoporous silica microspheres with mesostructured cellular foams (mcfs) were prepared by nh(3)/water vapor-induced internal hydrolysis method. zirconia layer coated mcf microspheres were characterized by sem, xrd, n(2) sorption, uv, and chromatographic analysis, and explored for enrichment of phosphopeptides. zro(2)/mcf microspheres in solid-phase extraction (spe) mode demonstrated much higher selectivity and higher efficiency towards phosphopeptide enrichment than bulk zro(2) particles. in particular, the selectivities of zro(2)/mcf microspheres towards multi-phosphopeptides are even higher than that of the widely used commercial tio(2) microparticles. the zro(2)/mcf microspheres were also applied to enrich endogenous phosphopeptides from human serum, and twelve endogenous phosphorylated peptides could be specifically enriched. (c) 2010 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | metal-oxide chromatography ; phosphorylated peptides ; mass-spectrometry ; phosphoproteome analysis ; affinity-chromatography ; titanium-dioxide ; protein-phosphorylation ; liquid-chromatography ; chemo-affinity ; scale |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000283903300012 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141634]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Biotech Dept, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Huihui,Yan, Jingyu,Yu, Long,et al. Zirconia layer coated mesoporous silica microspheres used for highly specific phosphopeptide enrichment[J]. talanta,2010,82(5):1701-1707. |
| APA | Wan, Huihui.,Yan, Jingyu.,Yu, Long.,Zhang, Xiuli.,Xue, Xingya.,...&Liang, Xinmiao.(2010).Zirconia layer coated mesoporous silica microspheres used for highly specific phosphopeptide enrichment.talanta,82(5),1701-1707. |
| MLA | Wan, Huihui,et al."Zirconia layer coated mesoporous silica microspheres used for highly specific phosphopeptide enrichment".talanta 82.5(2010):1701-1707. |
入库方式： OAI收割
来源：大连化学物理研究所
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