Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation
文献类型：期刊论文
| 作者 | Wang Ena1; Duan Shujing1; He Xiwen1; Chen Langxing1; Li Wenyou1; Zhang Yukui1,2 |
| 刊名 | acta chimica sinica
 |
| 出版日期 | 2010-12-14 |
| 卷号 | 68期号:23页码:2421-2428 |
| 关键词 | silica strong anion-exchange chromatography atom transfer radical polymerization protein's separation |
| 英文摘要 | a new strong anion-exchange stationary phase for hplc has been synthesized by surface-initiated atom transfer radical polymerization (atrp). firstly, 2-bromoisobutyryl bromide as the atrp initiator was attached onto the macroporous silica via chemical reaction. in the next stage, methacrylatoethyl trimethyl ammonium chloride (mata) carrying strong anion exchanger groups as the monomer was polymerized on the initiator-immobilized particles via surface-initiated atrp. after studying the adsorption properties, the suitable support was packed into stainless steel column to separate acid proteins by the linear gradient elution in 20 min at flow rate of 0.75 ml/min. the effects of flow rate, ph and organic solvents on the separation and retention ability of proteins were studied in detail. this support was also used for the separation and purification of ovalbumin from egg in only one step. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | stationary-phase ; surface ; silica ; brushes ; nanoparticles ; fabrication |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286082500008 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141654]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Ena,Duan Shujing,He Xiwen,et al. Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation[J]. acta chimica sinica,2010,68(23):2421-2428. |
| APA | Wang Ena,Duan Shujing,He Xiwen,Chen Langxing,Li Wenyou,&Zhang Yukui.(2010).Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation.acta chimica sinica,68(23),2421-2428. |
| MLA | Wang Ena,et al."Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation".acta chimica sinica 68.23(2010):2421-2428. |
入库方式： OAI收割
来源：大连化学物理研究所
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