Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum
文献类型：期刊论文
| 作者 | Gao, Liang1,2; Zhang, Jie1; Zhang, Weibing1,3; Shan, Yichu1; Liang, Zhen1; Zhang, Lihua1; Huo, Yushu1; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 33期号:23-24页码:3817-3821 |
| 关键词 | Comprehensive 2-D chromatography Normal phase LC Supercritical fluid chromatography Traditional Chinese medicines |
| 英文摘要 | in this study, a comprehensive 2-d chromatography was constructed, consisting of normal phase lc (nplc) with a cn column as the first dimension, and supercritical fluid chromatography (sfc), with a merck chromolith flash c(18) column as the second dimension, which were connected by a 10-port, dual-position valve controlled automatically by a self-designed software. such platform was applied into the analysis of the fruiting bodies of ganoderma lucidum, a traditional chinese medicine, and within 2 h analysis, the obtained peak capacity of the 2-d-nplc-sfc system was about 350, obviously higher than that of each dimension. these results demonstrate that 2-d-nplc-sfc is not only of good orthogonality, but also of high throughput for the analysis of complex samples. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | shotgun proteome analysis ; multidimensional separation ; mass-spectrometry ; reversed-phase ; column ; identification ; interface ; system ; rplc |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285312700026 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141675]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatograph Res & Anal Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.E China Univ Sci & Technol, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Liang,Zhang, Jie,Zhang, Weibing,et al. Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum[J]. journal of separation science,2010,33(23-24):3817-3821. |
| APA | Gao, Liang.,Zhang, Jie.,Zhang, Weibing.,Shan, Yichu.,Liang, Zhen.,...&Zhang, Yukui.(2010).Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum.journal of separation science,33(23-24),3817-3821. |
| MLA | Gao, Liang,et al."Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum".journal of separation science 33.23-24(2010):3817-3821. |
入库方式： OAI收割
来源：大连化学物理研究所
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