Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles
文献类型：期刊论文
| 作者 | Wang, Xinyi2; Xu, Yao1; Xu, Xiao1; Hu, Ke1; Xiang, Minghui1; Li, Limei2; Liu, Feng1; Li, Na1 |
| 刊名 | talanta
 |
| 出版日期 | 2010-07-15 |
| 卷号 | 82期号:2页码:693-697 |
| 关键词 | Lysozyme Plasmon Resonance Light-Scattering Gold Nanoparticles Urinary sample |
| 英文摘要 | the purpose of this study was to establish a simple and sensitive analytical method for lysozyme using plasmon resonance light-scattering (prls) technique with gold nanoparticles (aunps) as the probe. nanomolar level of lysozyme induced aunps aggregation with enhanced prls. for 1.4 nm citrate-capped aunps (13 nm in diameter), the linear range of the calibration curve was 15-50 nm with a detection limit of 13.1 nm for lysozyme. six nanomolar lysozyme can produce an observable prls enhancement. most potential interfering substances present in urine had a negligible effect on the determination. the interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at ph 4.8-4.9. the 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. this simple and sensitive method for lysozyme does not require sample clean-up and aunps modification, thus provided an alternative for urinary lysozyme determination. (c) 2010 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | linked-immunosorbent-assay ; human serum-albumin ; egg-white ; protein ; chromatography ; biosensor ; probe ; electrophoresis ; dysfunction ; aptamer |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280375700040 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141687]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Shenyang Agr Univ, Coll Sci, Shenyang 110161, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xinyi,Xu, Yao,Xu, Xiao,et al. Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles[J]. talanta,2010,82(2):693-697. |
| APA | Wang, Xinyi.,Xu, Yao.,Xu, Xiao.,Hu, Ke.,Xiang, Minghui.,...&Li, Na.(2010).Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles.talanta,82(2),693-697. |
| MLA | Wang, Xinyi,et al."Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles".talanta 82.2(2010):693-697. |
入库方式： OAI收割
来源：大连化学物理研究所
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