Construction of an Artificial Signal Transduction System on Bilayer Vesicles: Insertion of Transmembrane Peptide of Bacterial Histidine Protein Kinase on Bilayer Vesicles
文献类型：期刊论文
| 作者 | Quan Chunshan1; Ma Jinlong1,2; Wang Xue3; Li Wei4; Fan Shengdi1 |
| 刊名 | acta chimica sinica
 |
| 出版日期 | 2010-06-28 |
| 卷号 | 68期号:12页码:1167-1173 |
| 关键词 | transmembrane transduction Staphyloccocus aureus AgrC transmembrane insertion |
| 英文摘要 | one of the six transmembrane domains of agrc from staphylococcus aureus atcc 6538 (agr i type) which is closest with the process of transmembrane signal transduction in quorum sensing was selected as a receptor. this polypeptide was inserted into the artificial cell membrane by ultrasonic procedure, and the pattern of the vesicles was analyzed by transmission electron microscopy (tem) and atomic force microscopy (afm). it was found that the single shape of most vesicles (blank and embedded with peptide) was uniform sphere, and the diameter size is from 50 to 200 nm. considering the impact of suspension ph, concentration and temperature factors to the stability of the embeded system, it is found that the system was the most stable under the following conditions: ph 7.0, temperature lower than 30 degrees c, mole ratio of the polypeptide and n(+)c(5)ala2c(16) being 1 : 100. meanwhile, it is also discovered that polypeptide was capable of inserting into bilayer vesicles stably and positively to form embeded system by circular dichroism spectra and differential scanning calorimetry. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | staphylococcus-aureus ; enzymatic-activity ; intermolecular communication ; lipid signal ; receptor ; recognition ; membrane ; agrc ; activation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000279709400003 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141706]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Nationalities Univ, Coll Life Sci, Dalian 116600, Peoples R China 2.Zhejiang Univ, Dept Chem & Biol Engn, Hangzhou 310027, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 4.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian 116064, Peoples R China |
| 推荐引用方式 GB/T 7714 | Quan Chunshan,Ma Jinlong,Wang Xue,et al. Construction of an Artificial Signal Transduction System on Bilayer Vesicles: Insertion of Transmembrane Peptide of Bacterial Histidine Protein Kinase on Bilayer Vesicles[J]. acta chimica sinica,2010,68(12):1167-1173. |
| APA | Quan Chunshan,Ma Jinlong,Wang Xue,Li Wei,&Fan Shengdi.(2010).Construction of an Artificial Signal Transduction System on Bilayer Vesicles: Insertion of Transmembrane Peptide of Bacterial Histidine Protein Kinase on Bilayer Vesicles.acta chimica sinica,68(12),1167-1173. |
| MLA | Quan Chunshan,et al."Construction of an Artificial Signal Transduction System on Bilayer Vesicles: Insertion of Transmembrane Peptide of Bacterial Histidine Protein Kinase on Bilayer Vesicles".acta chimica sinica 68.12(2010):1167-1173. |
入库方式： OAI收割
来源：大连化学物理研究所
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