Novel asymmetric Cy5 dyes: Synthesis, photostabilities and high sensitivity in protein fluorescence labeling
文献类型：期刊论文
| 作者 | Wang, Li1; Fan, Jiangli1; Qiao, Xiaoqiang2; Peng, Xiaojun1; Dai, Bin1; Wang, Bingshuai1; Sun, Shiguo1; Zhang, Lihua2; Zhang, Yukui2 |
| 刊名 | journal of photochemistry and photobiology a-chemistry
 |
| 出版日期 | 2010-02-25 |
| 卷号 | 210期号:2-3页码:168-172 |
| 关键词 | Cy5 Cyanine dye Photophysical property Photostability Fluorescent labeling High performance liquid chromatography |
| 英文摘要 | several novel water-soluble asymmetric pentamethine cyanine dyes were synthesized. the maximum absorption and emission wavelengths of the dyes in different solvents were in the range from 647 to 665 nm and exhibited negative solvatochromism with increasing solvent polarity. the fluorescence quantum yields of the dyes were about 0.1 in water, and were obviously higher than those of hydrophobic cyanine dyes. dyes with n-benzyl groups and n-sulfo-groups displayed greater photostability than dyes with n-carboxypentyl groups in water. the limit of detection of dye 5a for bsa was 1.2 x 10(-8) mol l(-1) by high performance liquid chromatography with fluorescent director about 100-fold lower than that by uv detection (1.0 x 10(-6) mol l(-1)). therefore, dye 5a could be used to improve photostability and detection sensitivity in protein analysis. (c) 2010 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, physical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | quantum yields ; fluorochrome |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000276328100011 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141763]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Li,Fan, Jiangli,Qiao, Xiaoqiang,et al. Novel asymmetric Cy5 dyes: Synthesis, photostabilities and high sensitivity in protein fluorescence labeling[J]. journal of photochemistry and photobiology a-chemistry,2010,210(2-3):168-172. |
| APA | Wang, Li.,Fan, Jiangli.,Qiao, Xiaoqiang.,Peng, Xiaojun.,Dai, Bin.,...&Zhang, Yukui.(2010).Novel asymmetric Cy5 dyes: Synthesis, photostabilities and high sensitivity in protein fluorescence labeling.journal of photochemistry and photobiology a-chemistry,210(2-3),168-172. |
| MLA | Wang, Li,et al."Novel asymmetric Cy5 dyes: Synthesis, photostabilities and high sensitivity in protein fluorescence labeling".journal of photochemistry and photobiology a-chemistry 210.2-3(2010):168-172. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。