Properties of i-Motif Under Molecular Crowding Conditions
文献类型：期刊论文
| 作者 | Zhou Jun1,2; Jia Guo-Qing1,2; Feng Zhao-Chi1; Li Can1 |
| 刊名 | chemical journal of chinese universities-chinese
 |
| 出版日期 | 2010-02-10 |
| 卷号 | 31期号:2页码:309-311 |
| 关键词 | i-Motif Molecular crowding Polyethylene glycol(PEG) |
| 英文摘要 | i-motif is formed from two parallel duplexes by intercalating with each other in an antiparallel orientation and each duplex is held together via hemiprotonated cytosine(+)-cytosine base pairs. however, a cell is crowded with various biomolecules and little is known about the properties of i-motif under molecular crowding conditions until now. in the present study, we used human telomeric dna sequence, [c(3)ta(2)](3)c(3), as a model system to investigate such problem by circular dichroism( cd) and uv absorbance spectroscopy. based on the cd spectra, we found that there were no changes about the structure of i-motif in the presence of polyethylene glycol( peg). cd melting results showed that the thermal stability of i-motif was increased under molecular crowding conditions compare to that in dilute buffer, which was further demonstrated by uv-melting results. this work suggests that molecular crowding could not affect the structure of i-motif, which may depend on ph, while could enhance the thermal stability of i-motif. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | g-quadruplex ; dna ; sequences |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275476600019 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141770]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou Jun,Jia Guo-Qing,Feng Zhao-Chi,et al. Properties of i-Motif Under Molecular Crowding Conditions[J]. chemical journal of chinese universities-chinese,2010,31(2):309-311. |
| APA | Zhou Jun,Jia Guo-Qing,Feng Zhao-Chi,&Li Can.(2010).Properties of i-Motif Under Molecular Crowding Conditions.chemical journal of chinese universities-chinese,31(2),309-311. |
| MLA | Zhou Jun,et al."Properties of i-Motif Under Molecular Crowding Conditions".chemical journal of chinese universities-chinese 31.2(2010):309-311. |
入库方式： OAI收割
来源：大连化学物理研究所
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