A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis
文献类型：期刊论文
| 作者 | Pan, Xiaoyan1,2; Jiang, Lei1; Liu, Kaiying1; Lin, Bingcheng1; Qin, Jianhua1 |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2010-07-26 |
| 卷号 | 674期号:1页码:110-115 |
| 关键词 | Microfluidic Parallel analysis Multichamber polymerase chain reaction Pathogen detection Genotyping |
| 英文摘要 | this work describes a microfluidic device integrated with multichamber polymerase chain reaction (pcr) and multichannel separation for parallel genetic analysis. the microdevice consists of three functional units: temperature control, multiple pcr (four chambers pcr), and multiple channel separation (four separation channels, each channel connected to a pcr chamber). platinum (pt)/titanium (ti) microheater was used to ensure homogeneous temperature field, and pt-chip sensor was used for temperature monitoring. the interface between chip-pcr and chip separation was simplified by connecting the pcr chamber with separation channel directly. after chip-pcr, pcr products were introduced into parallel separation channels for subsequent separation/detection by applying an electric field automatically. this microdevice was successfully applied for detection of pathogens including hepatitis b virus (hbv) and mycobacterium tuberculosis (mtb), and genotyping of human leucocyte antigen (hla)-b27 as well, demonstrating the feasibility of the integrated microdevice for parallel genetic analysis. (c) 2010 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | capillary electrophoresis microsystem ; analysis system ; pcr amplification ; dna amplification ; chip ; microdevice ; extraction ; microchip ; samples |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000280570400016 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141790]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pan, Xiaoyan,Jiang, Lei,Liu, Kaiying,et al. A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis[J]. analytica chimica acta,2010,674(1):110-115. |
| APA | Pan, Xiaoyan,Jiang, Lei,Liu, Kaiying,Lin, Bingcheng,&Qin, Jianhua.(2010).A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis.analytica chimica acta,674(1),110-115. |
| MLA | Pan, Xiaoyan,et al."A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis".analytica chimica acta 674.1(2010):110-115. |
入库方式： OAI收割
来源：大连化学物理研究所
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