Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion
文献类型：期刊论文
| 作者 | Sun, Liangliang1,2; Ma, Junfeng1,2; Qiao, Xiaoqiang1,2; Liang, Yu1,2; Zhu, Guijie1; Shan, Yichu1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2010-03-15 |
| 卷号 | 82期号:6页码:2574-2579 |
| 英文摘要 | an integrated sample treatment device, composed of a membrane interface and a monolithic hybrid silica based immobilized enzymatic reactor (imer), was developed for the simultaneous sample buffer exchange, protein enrichment, and online digestion, by which for the sample buffer, the acetonitrile content was reduced to similar to 1/10 of the initial one, and the ph value was adjusted from similar to 3.0 to similar to 8.0, compatible for online trypsin digestion. furthermore, the signal intensity of myoglobin digests was improved by over 10 times. such an integrated device was successfully applied to the online treatment of three protein eluates obtained by reverse-phase liquid chromatography (rplc) separation, followed by further protein digest analysis with microreverse-phase liquid chromatography-electrospray ionization-tandem mass spectrometry (,mu rplc-esi-ms/ms). the experimental results showed that the performance of such an integrated sample treatment device was comparable to that of the traditional offline sample treatment method, including lyophilization and in-solution digestion. however, the consumed time was reduced to 1/192. all these results,demonstrate that such an integrated sample treatment device could be further online coupled with protein separation, peptide separation, and identification, to achieve high-throughput proteome analysis. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mass-spectrometry ; capillary-electrophoresis ; bottom-up ; top-down ; identification ; separation ; ms ; microdialysis ; microreactor ; proteomics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275379300056 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141801]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Liangliang,Ma, Junfeng,Qiao, Xiaoqiang,et al. Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion[J]. analytical chemistry,2010,82(6):2574-2579. |
| APA | Sun, Liangliang.,Ma, Junfeng.,Qiao, Xiaoqiang.,Liang, Yu.,Zhu, Guijie.,...&Zhang, Yukui.(2010).Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion.analytical chemistry,82(6),2574-2579. |
| MLA | Sun, Liangliang,et al."Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion".analytical chemistry 82.6(2010):2574-2579. |
入库方式： OAI收割
来源：大连化学物理研究所
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