Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis
文献类型：期刊论文
| 作者 | Ma, Junfeng1,2,3; Hou, Chunyan1,2,3; Sun, Liangliang1,2,3; Tao, Dingyin1,2,3; Zhang, Yanyan2,3; Shan, Yichu1,2; Liang, Zhen1,2; Zhang, Lihua1,2; Yang, Ling2; Zhang, Yukui1,2 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2010-12-01 |
| 卷号 | 82期号:23页码:9622-9625 |
| 英文摘要 | in this study, a facile system for membrane proteome profiling was established, in which membrane proteins were solubilized by formic acid, online digested by a pepsin-based immobilized enzyme reactor (pepsin imer), and analyzed by strong cation exchange and microflow reversed phase liquid chromatography with electrospray ionization tandem mass spectrometry (scx-mu rplc-esi-ms/ms) under optimized conditions, such a system showed excellent compatibility between all crucial steps and was successfully applied for analyzing integral membrane proteins extracted from rat liver microsomes out of the 235 unique proteins positively identified, 39% (91/235) were annotated as membrane proteins with one or more transmembrane domains (tmds) it is anticipated that the efficient sample treatment and the relevant online analytical system might provide a promising tool for automated and comprehensive profiling of membrane proteomes |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | proteins ; ms/ms ; identification ; surfactant ; ms |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284668600007 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141823]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Junfeng,Hou, Chunyan,Sun, Liangliang,et al. Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis[J]. analytical chemistry,2010,82(23):9622-9625. |
| APA | Ma, Junfeng.,Hou, Chunyan.,Sun, Liangliang.,Tao, Dingyin.,Zhang, Yanyan.,...&Zhang, Yukui.(2010).Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis.analytical chemistry,82(23),9622-9625. |
| MLA | Ma, Junfeng,et al."Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis".analytical chemistry 82.23(2010):9622-9625. |
入库方式： OAI收割
来源：大连化学物理研究所
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