Determination of diketopiperazines of Burkholderia cepacia CF-66 by gas chromatography-mass spectrometry
文献类型：期刊论文
| 作者 | Wang, Jian-Hua2,3; Quan, Chun-Shan1; Qi, Xiao-Hui1; Li, Xin2,3; Fan, Sheng-Di1 |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2010-03-01 |
| 卷号 | 396期号:5页码:1773-1779 |
| 关键词 | Quorum sensing Signal molecules Burkholderia cepacia CF-66 Gas chromatography-mass spectrometry Polymerase chain reaction Diketopiperazines |
| 英文摘要 | bacteria communicate with each other by a process termed "quorum sensing" (qs), and diffusible, low-molecular-weight chemicals, called signal molecules, are used as the communication languages. in cell-free burkholderia cepacia cf-66 culture supernatants, five compounds suspected of being signal molecules were identified. the gene (cepi) related with ahls synthesis were not detected by polymerase chain reaction (pcr) using specific primers. gas chromatography-mass spectrometry (gc-ms) revealed that these compounds were not ahls but the diketopiperazines (dkps) cyclo(prophe), cyclo(pro-tyr), cyclo(ala-val), cyclo(pro-leu), and cyclo(pro-val), all of which were both d and l-type. four kinds of dkps had been isolated from other gramnegative bacteria, but the other was a novel kind discovered in cf-66, and l-cyclo (pro-phe) was quantified by gc-ms. it was found that exogenous dkps had a negative effect on the candidacidal activity of the culture supernatant extracts. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | lactone signal molecules ; gram-negative bacteria ; pseudomonas-aeruginosa ; homoserine lactones ; antifungal activity ; cyclic dipeptides ; cross-talk ; quorum ; identification ; communication |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274742900019 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141878]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Nationalities Univ, Life Sci Coll, Dalian 116600, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jian-Hua,Quan, Chun-Shan,Qi, Xiao-Hui,et al. Determination of diketopiperazines of Burkholderia cepacia CF-66 by gas chromatography-mass spectrometry[J]. analytical and bioanalytical chemistry,2010,396(5):1773-1779. |
| APA | Wang, Jian-Hua,Quan, Chun-Shan,Qi, Xiao-Hui,Li, Xin,&Fan, Sheng-Di.(2010).Determination of diketopiperazines of Burkholderia cepacia CF-66 by gas chromatography-mass spectrometry.analytical and bioanalytical chemistry,396(5),1773-1779. |
| MLA | Wang, Jian-Hua,et al."Determination of diketopiperazines of Burkholderia cepacia CF-66 by gas chromatography-mass spectrometry".analytical and bioanalytical chemistry 396.5(2010):1773-1779. |
入库方式： OAI收割
来源：大连化学物理研究所
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