Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide
文献类型：期刊论文
| 作者 | Xu, Qingsong1,2; Ma, Pan1,2; Yu, Weiting1; Tan, Chengyu3; Liu, Hongtao1,2; Xiong, Chuannan1,2; Qiao, Ying1,2; Du, Yuguang1 |
| 刊名 | marine biotechnology
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 12期号:3页码:292-298 |
| 关键词 | Chitooligosaccharides Human embryonic hepatocytes Oxidative stress Apoptosis |
| 英文摘要 | chitooligosaccharides (cos) has many biological activities, such as antitumor activity and hepatoprotective effect. herein, we investigated the protective effect of cos against hydrogen peroxide (h(2)o(2))-induced oxidative stress on human embryonic hepatocytes (l02 cells) and its scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical in vitro. the results showed that the lost cell viability induced by h(2)o(2) was markedly restored after 24 h pre-incubation with cos (0.1-0.4 mg/ml). this rescue effect could be related to the antioxidant property of cos, in which we showed that the radical scavenging activity of cos reached 80% at concentration of 2 mg/ml. in addition, cos could prevent cell apoptosis induced by h(2)o(2), as shown by the inhibition of the cleavage of poly (adenosine diphosphate-ribose) polymerase and increased expression of the anti-apoptotic protein bcl-xl. furthermore, we have utilized confocal laser microscopy to observe cellular uptake of cos, an important step for cos to exert its effects on target cells. taken together, our findings suggested that cos could effectively protect l02 cells against oxidative stress, which might be useful in clinical setting during the treatment of oxidative stress-related liver damages. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology ; marine & freshwater biology |
| 研究领域[WOS] | biotechnology & applied microbiology ; marine & freshwater biology |
| 关键词[WOS] | antioxidant activities ; in-vitro ; chitosan oligosaccharide ; induced apoptosis ; molecular-weight ; mechanisms ; pathway ; cells ; mice ; oligochitosan |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278095400007 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141879]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Qingsong,Ma, Pan,Yu, Weiting,et al. Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide[J]. marine biotechnology,2010,12(3):292-298. |
| APA | Xu, Qingsong.,Ma, Pan.,Yu, Weiting.,Tan, Chengyu.,Liu, Hongtao.,...&Du, Yuguang.(2010).Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide.marine biotechnology,12(3),292-298. |
| MLA | Xu, Qingsong,et al."Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide".marine biotechnology 12.3(2010):292-298. |
入库方式： OAI收割
来源：大连化学物理研究所
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