Molecular Cloning and Characterization of a Brassica napus L. MAP Kinase Involved in Oligochitosan-Induced Defense Signaling
文献类型：期刊论文
| 作者 | Yin, Heng; Zhao, Xiaoming; Bai, Xuefang; Du, Yuguang |
| 刊名 | plant molecular biology reporter
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 28期号:2页码:292-301 |
| 关键词 | Oligochitosan Brassica napus BnOIPK Plant defense Signal transduction |
| 英文摘要 | oligochitosan is a potent plant defense elicitor. in this paper, a novel brassica napus mitogen-activated protein kinase (mapk) gene induced by oligochitosan was isolated by rapid amplification of cdna ends technique and designated as bnoipk (oligochitosan-induced protein kinase (oipk)). bnoipk, with high sequence similarity to previously reported plant mapk genes, encodes a 373-amino acid protein and belongs to the b subgroup of plant mapk. bioinformatics analysis showed bnoipk contains one phosphorylation motif (tey) and a conserved common docking domain in its c-terminal extension. with a constitutive expression in seedling leaves, bnoipk is further upregulated upon treatment with plant hormone jasmonic acid (ja), but did not markedly respond to salicylic acid and abscisic acid. activation of bnoipk transcripts induced by oligochitosan depending on nitric oxide (no) and hydrogen peroxide (h(2)o(2)) was identified by using no, h(2)o(2), and their scavenger, respectively. polyclonal antibody bok was prepared by using a 69-kd gst-bnoipk fusion protein which was produced from pgex-4t-1 vector. western-blot assays showed bnoipk could be induced by oligochitosan and ja at a short time. all these results suggest that bnoipk might be a key node of ja-mediated defense signaling and act on the downstream of no and h(2)o(2). |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemical research methods ; plant sciences |
| 研究领域[WOS] | biochemistry & molecular biology ; plant sciences |
| 关键词[WOS] | activated protein-kinases ; environmental-stresses ; abscisic-acid ; chitosan ; arabidopsis ; resistance ; gene ; pathway ; rice ; responses |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274961000012 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141919]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Heng,Zhao, Xiaoming,Bai, Xuefang,et al. Molecular Cloning and Characterization of a Brassica napus L. MAP Kinase Involved in Oligochitosan-Induced Defense Signaling[J]. plant molecular biology reporter,2010,28(2):292-301. |
| APA | Yin, Heng,Zhao, Xiaoming,Bai, Xuefang,&Du, Yuguang.(2010).Molecular Cloning and Characterization of a Brassica napus L. MAP Kinase Involved in Oligochitosan-Induced Defense Signaling.plant molecular biology reporter,28(2),292-301. |
| MLA | Yin, Heng,et al."Molecular Cloning and Characterization of a Brassica napus L. MAP Kinase Involved in Oligochitosan-Induced Defense Signaling".plant molecular biology reporter 28.2(2010):292-301. |
入库方式： OAI收割
来源：大连化学物理研究所
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