Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system
文献类型：期刊论文
| 作者 | Yang, Qin; Shi, Xianzhe; Wang, Yuan; Wang, Wenzhao; He, Hongbin; Lu, Xin; Xu, Guowang |
| 刊名 | journal of separation science
 |
| 出版日期 | 2010-05-01 |
| 卷号 | 33期号:10页码:1495-1503 |
| 关键词 | Column switching Hydrophilic interaction chromatography Lung cancer Metabonomics Two-dimensional LC |
| 英文摘要 | lung cancer is one of the most common and lethal cancers in the world. in this study, a home-devised hydrophilic interaction chromatography/rplc-ms (hilic/rplc-ms) system was developed to study the urinary metabonomics of lung cancer patients. this system combined the orthogonal selectivity of hilic and rplc and could chromatographically reveal more comprehensive information of the urinary metabolites. within a total analysis time of 50 min, we detected 577 polar metabolite ions on the first hilic column and 261 apolar ones on the second rplc column. in addition, an orthogonal signal correction partial least-squares discriminant analysis model was constructed to characterize differences between health and lung cancer cases. eleven potential biomarkers, ten from hilic column and one from the second rp column, were identified and all of these biomarkers were found upregulated in lung cancer patients. overall, the results indicated that the developed hilic/rplc-ms system is a promising tool for metabonomic studies in revealing more information of highly complex samples. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | liquid chromatography/mass spectrometry ; mass-spectrometry ; magnetic-resonance ; rat urine ; tof-ms ; phase ; hplc ; acid ; identification ; spectroscopy |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000278572000017 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141947]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Qin,Shi, Xianzhe,Wang, Yuan,et al. Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system[J]. journal of separation science,2010,33(10):1495-1503. |
| APA | Yang, Qin.,Shi, Xianzhe.,Wang, Yuan.,Wang, Wenzhao.,He, Hongbin.,...&Xu, Guowang.(2010).Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system.journal of separation science,33(10),1495-1503. |
| MLA | Yang, Qin,et al."Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system".journal of separation science 33.10(2010):1495-1503. |
入库方式： OAI收割
来源：大连化学物理研究所
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