Size-exclusion separation of proteins using a biocompatible polymeric monolithic capillary column with mesoporosity
文献类型：期刊论文
| 作者 | Li, Yun1,2; Tolley, H. Dennis3; Lee, Milton L.1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2010-12-24 |
| 卷号 | 1217期号:52页码:8181-8185 |
| 关键词 | Monolithic columns Liquid chromatography Capillary columns Size exclusion chromatography Pore size distribution Porosity Biocompatible Polyethylene glycol diacrylate Brij Proteins Peptides |
| 英文摘要 | biocompatible poly(ethylene glycol methyl ether acrylate-co-polyethylene glycol diacrylate) monoliths were prepared for size exclusion chromatography (sec) of proteins in the capillary format using brij 58p in a mixture of hexanes and dodecanol as porogens. the monolithic columns provided size separation of four proteins in 20 mm sodium phosphate buffer (ph 7.0) containing 0.15 m nacl, and there was a linear relationship between the retention times and the logarithmic values of the molecular weights. compared to sec monoliths previously synthesized using a triblock copolymer of polyethylene oxide and polypropylene oxide, an increase in mesoporosity was confirmed by inverse size exclusion chromatography. as a result, improved protein separation in the high molecular weight range and reduced column back-pressure were observed. (c) 2010 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | hydrophobic interaction chromatography ; hydrophilic interaction chromatography ; liquid-chromatography ; peptides ; rod |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285899400013 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141969]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China 3.Brigham Young Univ, Dept Stat, Provo, UT 84602 USA |
| 推荐引用方式 GB/T 7714 | Li, Yun,Tolley, H. Dennis,Lee, Milton L.. Size-exclusion separation of proteins using a biocompatible polymeric monolithic capillary column with mesoporosity[J]. journal of chromatography a,2010,1217(52):8181-8185. |
| APA | Li, Yun,Tolley, H. Dennis,&Lee, Milton L..(2010).Size-exclusion separation of proteins using a biocompatible polymeric monolithic capillary column with mesoporosity.journal of chromatography a,1217(52),8181-8185. |
| MLA | Li, Yun,et al."Size-exclusion separation of proteins using a biocompatible polymeric monolithic capillary column with mesoporosity".journal of chromatography a 1217.52(2010):8181-8185. |
入库方式： OAI收割
来源：大连化学物理研究所
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