Molecular Cloning and Characterization of a Malic Enzyme Gene from the Oleaginous Yeast Lipomyces starkeyi
文献类型：期刊论文
| 作者 | Tang, Wei1,2; Zhang, Sufang1; Tan, Haidong1; Zhao, Zongbao K.1 |
| 刊名 | molecular biotechnology
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 45期号:2页码:121-128 |
| 关键词 | Lipomyces starkeyi Malic enzyme RACE Protein expression Enzyme kinetics |
| 英文摘要 | the malic enzyme-encoding cdna (gq372891) from the oleaginous yeast lipomyces starkeyi as 2.1560 was isolated, which has an 1719-bp open reading frame flanked by a 290-bp 5' untranslated sequence and a 92-bp 3' untranslated sequence. the proposed gene, lsme1, encoded a protein with 572 amino acid residues. the protein presented 58% sequence identity with the malic enzymes from yarrowia lipolytica clib122 and aspergillus fumigatus af293. the lsme1 gene was cloned into the vector pmal-p4x to express a fusion protein (mbp-lsme1) in escherichia coli tb1. the fusion protein was purified and then cleaved by factor xa to give the recombinant lsme1. this purified enzyme took either nad(+) or nadp(+) as the coenzyme but preferred nad(+). the k (m) values for malic acid, nad(+) and nadp(+) were 0.85 +/- a 0.05 mm, 0.34 +/- a 0.08 mm, and 7.4 +/- a 0.32 mm, respectively, at ph 7.3. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 关键词[WOS] | lipid-accumulation ; isocitrate dehydrogenase ; saccharomyces-cerevisiae ; pigeon liver ; purification ; metabolism ; acid ; expression ; overexpression ; proteins |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000277343900004 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/141999]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Wei,Zhang, Sufang,Tan, Haidong,et al. Molecular Cloning and Characterization of a Malic Enzyme Gene from the Oleaginous Yeast Lipomyces starkeyi[J]. molecular biotechnology,2010,45(2):121-128. |
| APA | Tang, Wei,Zhang, Sufang,Tan, Haidong,&Zhao, Zongbao K..(2010).Molecular Cloning and Characterization of a Malic Enzyme Gene from the Oleaginous Yeast Lipomyces starkeyi.molecular biotechnology,45(2),121-128. |
| MLA | Tang, Wei,et al."Molecular Cloning and Characterization of a Malic Enzyme Gene from the Oleaginous Yeast Lipomyces starkeyi".molecular biotechnology 45.2(2010):121-128. |
入库方式： OAI收割
来源：大连化学物理研究所
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