Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells
文献类型：期刊论文
| 作者 | Ye, Nannan1; Wang, Ming-Wei2; Qin, Jianhua1; Lin, Bingcheng1 |
| 刊名 | biomedical microdevices
 |
| 出版日期 | 2010-06-01 |
| 卷号 | 12期号:3页码:513-521 |
| 关键词 | Microfluidic Formyl peptide receptor Chemotaxis Calcium mobilization FPR ligand uptake |
| 英文摘要 | the human formyl peptide receptor (fpr) plays an important role in inflammation and immunity. finding of specific agonists and antagonists of fpr may provide potential therapeutic agents for fpr related disorders. the binding of agonist by fpr induces a cascade of g protein-mediated signaling events leading to neutrophil chemotaxis, intracellualr calcium mobilization, fpr ligand uptake and so on. this work proposed a microfluidic-based method to characterize fpr-related cellular events in response to small peptides, n-formyl-met-leu-phe (fmlf), in rat basophilic leukemia cell line rbl-2h3 expressing human fpr (rbl-fpr). the results showed that fmlf triggered chemotaxis, calcium mobilization and fpr ligand uptake in rbl-fpr cells, indicating the potential role of fpr agonist. the chemotaxis index and the calcium mobilization intensity increased but the time course of calcium mobilization decreased, as the rising of fmlf concentration. the basic agreement between the microfluidic results and the previous studies demonstrated good feasibility of the microfluidic method for characterization of fpr agonist. microfluidic technology displays significant advantages over traditional methods in terms of sample consumption and assay time. it also facilitates experimental process and real-time observation of cellular responses at single cell resolution. |
| WOS标题词 | science & technology ; technology |
| 类目[WOS] | engineering, biomedical ; nanoscience & nanotechnology |
| 研究领域[WOS] | engineering ; science & technology - other topics |
| 关键词[WOS] | quantum dots ; chemotactic agonist ; tyr-met ; glutathione ; formyl-peptide-receptor-like-1 ; activation ; expression ; apoptosis ; binding ; system |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000277003600016 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142049]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.Natl Ctr Drug Screening, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Nannan,Wang, Ming-Wei,Qin, Jianhua,et al. Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells[J]. biomedical microdevices,2010,12(3):513-521. |
| APA | Ye, Nannan,Wang, Ming-Wei,Qin, Jianhua,&Lin, Bingcheng.(2010).Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells.biomedical microdevices,12(3),513-521. |
| MLA | Ye, Nannan,et al."Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells".biomedical microdevices 12.3(2010):513-521. |
入库方式： OAI收割
来源：大连化学物理研究所
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