Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles
文献类型：期刊论文
| 作者 | Chen, Qiang; Tang, Wei; Wang, Dingzhong; Wu, Xiaojie; Li, Na; Liu, Feng |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2010-10-15 |
| 卷号 | 26期号:2页码:575-579 |
| 关键词 | Aptamer Gold nanoparticles Amplification QCM-D biosensor Human alpha-thrombin |
| 英文摘要 | a highly sensitive quartz crystal microbalance with dissipation monitoring (qcm-d) biosensor for protein was developed using aptamer-functionalized gold nanoparticles (apt-gnps) for amplification. human alpha-thrombin, an important physiological protease found in blood, was chosen as the target protein. captured by immobilized aptamers, thrombin was determined on-line using apt-gnps to enhance both frequency and dissipation signals. the fabricated sandwich of aptamer/thrombin/apt-gnps on chip surface was confirmed by atomic force microscopy (afm). compared to direct assay, the detection limit for thrombin was down to 0.1 nm, yielding about 2 orders of magnitude improvement in sensitivity. this aptamer-based qcm-d biosensor also showed good selectivity and repeatability in complex matrix. for the first time, the dual-signal enhancement of apt-gnps on qcm-d sensing was demonstrated, and such design could provide a promising detection strategy for proteins with two binding sites. (c) 2010 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry, analytical ; electrochemistry ; nanoscience & nanotechnology |
| 研究领域[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry ; electrochemistry ; science & technology - other topics |
| 关键词[WOS] | human thrombin ; dna ; ligands ; bind ; rna ; molecules ; selection ; sensors ; signal |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000283804400043 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142207]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Qiang,Tang, Wei,Wang, Dingzhong,et al. Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles[J]. biosensors & bioelectronics,2010,26(2):575-579. |
| APA | Chen, Qiang,Tang, Wei,Wang, Dingzhong,Wu, Xiaojie,Li, Na,&Liu, Feng.(2010).Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles.biosensors & bioelectronics,26(2),575-579. |
| MLA | Chen, Qiang,et al."Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles".biosensors & bioelectronics 26.2(2010):575-579. |
入库方式： OAI收割
来源：大连化学物理研究所
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