中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA

文献类型:期刊论文

作者Gai, Qingqing1; Qu, Feng1; Zhang, Yukui2
刊名separation science and technology
出版日期2010
卷号45期号:16页码:2394-2399
关键词bovine hemoglobin bovine serum albumin molecularly imprinted gel polymer
英文摘要molecularly imprinted gel polymer (mip) for the selective imprinting of bovine hemoglobin (bhb) was prepared in aqueous media by bulk polymerization using polyacrylamide matrix. the synthesis conditions of bhb-mip were investigated, which involved the interaction of functional monomers and template protein in different molar ratio, solution ph, and ionic strength. the adsorption experiments indicated that bhb-mip had a high affinity for bhb over the non-imprinted polymers. the selectivity of bhb-mip for bhb and bovine serum albumin (bsa) with similar molecular weight was compared. it was demonstrated that bhb-mip had better selective adsorption and recognition properties to bhb especially in the presence of bsa as competing protein. it might be helpful for selectively separating template protein with mip from the proteins mixture with similar molecular weight.
WOS标题词science & technology ; physical sciences ; technology
类目[WOS]chemistry, multidisciplinary ; engineering, chemical
研究领域[WOS]chemistry ; engineering
关键词[WOS]electrostatic functional-groups ; solid-phase extraction ; bovine serum-albumin ; submicrometer particles ; protein recognition ; chitosan beads ; separation ; polymerization ; chromatography ; hemoglobin
收录类别SCI
语种英语
WOS记录号WOS:000284768500017
公开日期2015-11-17
源URL[http://159.226.238.44/handle/321008/142229]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式
GB/T 7714
Gai, Qingqing,Qu, Feng,Zhang, Yukui. The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA[J]. separation science and technology,2010,45(16):2394-2399.
APA Gai, Qingqing,Qu, Feng,&Zhang, Yukui.(2010).The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA.separation science and technology,45(16),2394-2399.
MLA Gai, Qingqing,et al."The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA".separation science and technology 45.16(2010):2394-2399.

入库方式: OAI收割

来源:大连化学物理研究所

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