The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA
文献类型:期刊论文
作者 | Gai, Qingqing1; Qu, Feng1; Zhang, Yukui2 |
刊名 | separation science and technology
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出版日期 | 2010 |
卷号 | 45期号:16页码:2394-2399 |
关键词 | bovine hemoglobin bovine serum albumin molecularly imprinted gel polymer |
英文摘要 | molecularly imprinted gel polymer (mip) for the selective imprinting of bovine hemoglobin (bhb) was prepared in aqueous media by bulk polymerization using polyacrylamide matrix. the synthesis conditions of bhb-mip were investigated, which involved the interaction of functional monomers and template protein in different molar ratio, solution ph, and ionic strength. the adsorption experiments indicated that bhb-mip had a high affinity for bhb over the non-imprinted polymers. the selectivity of bhb-mip for bhb and bovine serum albumin (bsa) with similar molecular weight was compared. it was demonstrated that bhb-mip had better selective adsorption and recognition properties to bhb especially in the presence of bsa as competing protein. it might be helpful for selectively separating template protein with mip from the proteins mixture with similar molecular weight. |
WOS标题词 | science & technology ; physical sciences ; technology |
类目[WOS] | chemistry, multidisciplinary ; engineering, chemical |
研究领域[WOS] | chemistry ; engineering |
关键词[WOS] | electrostatic functional-groups ; solid-phase extraction ; bovine serum-albumin ; submicrometer particles ; protein recognition ; chitosan beads ; separation ; polymerization ; chromatography ; hemoglobin |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000284768500017 |
公开日期 | 2015-11-17 |
源URL | [http://159.226.238.44/handle/321008/142229] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China |
推荐引用方式 GB/T 7714 | Gai, Qingqing,Qu, Feng,Zhang, Yukui. The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA[J]. separation science and technology,2010,45(16):2394-2399. |
APA | Gai, Qingqing,Qu, Feng,&Zhang, Yukui.(2010).The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA.separation science and technology,45(16),2394-2399. |
MLA | Gai, Qingqing,et al."The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA".separation science and technology 45.16(2010):2394-2399. |
入库方式: OAI收割
来源:大连化学物理研究所
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