Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay
文献类型：期刊论文
| 作者 | Xiao, Jing1,2; Zhang, Ying1; Wang, Jianzheng1,2; Yu, Weiting1; Wang, Wei1; Ma, Xiaojun1 |
| 刊名 | applied biochemistry and biotechnology
 |
| 出版日期 | 2010-11-01 |
| 卷号 | 162期号:7页码:1996-2007 |
| 关键词 | Microencapsulated cell APA Resazurin MTT Non-invasive monitoring of cell proliferation |
| 英文摘要 | cell microencapsulation is a promising approach for cell implantation, cell-based gene therapy and large-scale cell culture. for better quality control, it is important to accurately measure the microencapsulated cell viability and proliferation in the culture. a number of assays have been used for this purpose, but limitations arise. in this study, we investigated the feasibility and reliability of resazurin as a cell growth indicator in microencapsulated culture system. according to the experiment data, there was a reversible, time- and dose-dependent growth inhibition as observed for resazurin application in encapsulated cells. a positive relationship was observed between reduction of resazurin and cho cell number in microcapsule. moreover, the resazurin assay provided an equivalent result to the commonly used mtt method in determining cho cell proliferation in apa microcapsule with no notable influence on cell distribution and organization pattern. in conclusion, resazurin assay is offered as a simple, rapid and non-invasive method for in vitro microencapsulated cell viability and proliferation measurement. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 关键词[WOS] | mesenchymal stem-cells ; in-vitro ; alamarblue assay ; bioluminescence ; metabolism ; cultures ; growth ; tumor ; model ; blue |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000282315100015 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142231]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Lab Biomed Mat Engn, Dept Sci & Technol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Jing,Zhang, Ying,Wang, Jianzheng,et al. Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay[J]. applied biochemistry and biotechnology,2010,162(7):1996-2007. |
| APA | Xiao, Jing,Zhang, Ying,Wang, Jianzheng,Yu, Weiting,Wang, Wei,&Ma, Xiaojun.(2010).Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay.applied biochemistry and biotechnology,162(7),1996-2007. |
| MLA | Xiao, Jing,et al."Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay".applied biochemistry and biotechnology 162.7(2010):1996-2007. |
入库方式： OAI收割
来源：大连化学物理研究所
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