Production of two intermediate taxoids, 2-hydroxy-5 alpha, 10 beta-diacetoxytaxadiene and 2-hydroxy-5 alpha, 10 beta, 14 beta-triacetoxytaxadiene, from Taxus chinensis cell culture
文献类型：期刊论文
| 作者 | Gao, Ming-Bo1,2,3; Zhang, Wei1,4; Yue, Xiang-Guo1,2; Xiao, Hong-Bin5 |
| 刊名 | african journal of biotechnology
 |
| 出版日期 | 2010-12-06 |
| 卷号 | 9期号:49页码:8486-8491 |
| 关键词 | Taxus chinensis taxuyunnanine C methyl jasmonate 2 in situ absorption 2-hydroxy-5 alpha 2-hydroxy-5 alpha 3-dihydroxypropyl jasmonate 10 beta-diacetoxytaxadiene 10 beta 14 beta-triacetoxytaxadiene |
| 英文摘要 | the production of taxuyunnanine c (tc) (2 alpha, 5 alpha, 10 beta, 14 beta-tetraacetoxytaxa-4(20), 11(12)-diene) was significantly enhanced when taxus chinensis cell cultures were treated with methyl jasmonate (mja) or 2,3-dihydroxypropyl jasmonate (dhpja), a newly synthesized jasmonate analog, in combination with in situ absorption. along with the production of tc, two intermediate taxoids were produced and purified. on the basis of liquid chromatography-mass spectrometry (lc-ms) and nuclear magnetic resonance (nmr) spectral data, the chemical structures of the two intermediate taxoids were found to be 2-hydroxy-5 alpha, 10 beta-diacetoxytaxadiene and 2-hydroxy-5 alpha, 10 beta, 14 beta-triacetoxytaxadiene. this is the first study to report the production of these two taxoids using plant cell culture. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | 1st oxygenation step ; suspension-cultures ; geranylgeranyl diphosphate ; paclitaxel production ; taxane diterpenoids ; taxadiene synthase ; methyl jasmonate ; committed step ; biosynthesis ; expression |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285650000033 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142256]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100089, Peoples R China 3.Dalian Nationalities Univ, SEAC ME, Key Lab Biochem Engn, Dalian 116600, Peoples R China 4.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Mol Bioproc & Bioprod Lab, Adelaide, SA 5042, Australia 5.Chinese Acad Sci, Dalian Inst Chem Phys, Med Chem Grp, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Ming-Bo,Zhang, Wei,Yue, Xiang-Guo,et al. Production of two intermediate taxoids, 2-hydroxy-5 alpha, 10 beta-diacetoxytaxadiene and 2-hydroxy-5 alpha, 10 beta, 14 beta-triacetoxytaxadiene, from Taxus chinensis cell culture[J]. african journal of biotechnology,2010,9(49):8486-8491. |
| APA | Gao, Ming-Bo,Zhang, Wei,Yue, Xiang-Guo,&Xiao, Hong-Bin.(2010).Production of two intermediate taxoids, 2-hydroxy-5 alpha, 10 beta-diacetoxytaxadiene and 2-hydroxy-5 alpha, 10 beta, 14 beta-triacetoxytaxadiene, from Taxus chinensis cell culture.african journal of biotechnology,9(49),8486-8491. |
| MLA | Gao, Ming-Bo,et al."Production of two intermediate taxoids, 2-hydroxy-5 alpha, 10 beta-diacetoxytaxadiene and 2-hydroxy-5 alpha, 10 beta, 14 beta-triacetoxytaxadiene, from Taxus chinensis cell culture".african journal of biotechnology 9.49(2010):8486-8491. |
入库方式： OAI收割
来源：大连化学物理研究所
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