Vinyl functionalized silica hybrid monolith-based trypsin microreactor for on line digestion and separation via thiol-ene "click" strategy
文献类型：期刊论文
| 作者 | Chen, Yingzhuang1,3; Wu, Minghuo2; Wang, Keyi1; Chen, Bo3; Yao, Shouzhuo1,3; Zou, Hanfa2; Nie, Lihua1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2011-11-04 |
| 卷号 | 1218期号:44页码:7982-7988 |
| 关键词 | Vinyl functionalized Silica hybrid monolith Enzyme microreactor On-line digestion and separation Thiol-ene "click" reaction |
| 英文摘要 | a novel thiol-ene "click" strategy for the preparation of monolithic trypsin microreactor was proposed. the hybrid organic-inorganic monolithic capillary column with ene-functionality was fabricated by sol-gel process using tetramethoxysilane (tmos) and gamma-methacryloxypropyltrimethoxysilane (gamma-maps) as precursors. the disulfide bonds of trypsin were reduced to form free thiol groups. then the trypsin containing free thiol groups was attached on the gamma-maps hybrid monolithic column with ene-functionality via thiol-ene click chemistry to form a trypsin microreactor. the activity of the trypsin microreactor was characterized by detecting the substrate (n alpha-p-tosyl-l-arginine methyl ester hydrochloride. tame) and the product (n alpha-p-tosyl-l-arginine, ta) with on-line capillary zone electrophoresis. after investigating various synthesizing conditions, it was found that the microreactor with poly(n,n'-methylenebisacrylamide) as spacer can deliver the highest activity, yielding a rapid reaction rate. after repeatedly sampling and analyzing for 100 times, the monolithic trypsin microreactor still remained 87.5% of its initial activity. it was demonstrated that thiol-ene "click" strategy for the construction of enzyme microreactor is a promising method for the highly selective immobilization of proteins under mild conditions, especially enzymes with free thiol radicals. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | induced fluorescence detection ; immobilized enzyme reactors ; capillary-electrophoresis ; proteome analysis ; chemistry ; hydrogels ; ms/ms ; spectrometry ; reactivity ; surface |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296549000010 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142290]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China 2.CAS, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Hunan Normal Univ, Minist Educ, Key Lab Chem Biol & Tradit Chinese Med Res, Changsha 410081, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yingzhuang,Wu, Minghuo,Wang, Keyi,et al. Vinyl functionalized silica hybrid monolith-based trypsin microreactor for on line digestion and separation via thiol-ene "click" strategy[J]. journal of chromatography a,2011,1218(44):7982-7988. |
| APA | Chen, Yingzhuang.,Wu, Minghuo.,Wang, Keyi.,Chen, Bo.,Yao, Shouzhuo.,...&Nie, Lihua.(2011).Vinyl functionalized silica hybrid monolith-based trypsin microreactor for on line digestion and separation via thiol-ene "click" strategy.journal of chromatography a,1218(44),7982-7988. |
| MLA | Chen, Yingzhuang,et al."Vinyl functionalized silica hybrid monolith-based trypsin microreactor for on line digestion and separation via thiol-ene "click" strategy".journal of chromatography a 1218.44(2011):7982-7988. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。