Protein separation on a polar-copolymerized C-8 stationary phase
文献类型：期刊论文
| 作者 | Dong, Jun; Yu, Long; Zhang, Xiuli; Xue, Xingya; Guo, Zhimou; Liang, Xinmiao |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 399期号:10页码:3415-3421 |
| 关键词 | Reversed-phase liquid chromatography Polar-copolymerized Protein separation Glycoprotein |
| 英文摘要 | a novel polar-copolymerized c-8 stationary phase composed of octyl and chloropropyl (c-8-c3cl)-bonded silica has been developed for protein separation. separation of standard proteins on this homemade column was investigated and compared with separation on a waters symmetry 300 c-4 column. results indicated that the chromatographic performance of the homemade column in the separation of proteins was excellent. the new polar copolymerized c-8 stationary phase enabled glycoprotein separation with good resolution and reproducibility. it was also used for purification of ovalbumin glycoprotein in order to improve identification of the protein. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | hydrophobic interaction chromatography ; performance liquid-chromatography ; ion-exchange chromatography ; low ph stability ; affinity-chromatography ; silica ; glycoproteins ; proteomics ; systems |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288398700011 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142312]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Jun,Yu, Long,Zhang, Xiuli,et al. Protein separation on a polar-copolymerized C-8 stationary phase[J]. analytical and bioanalytical chemistry,2011,399(10):3415-3421. |
| APA | Dong, Jun,Yu, Long,Zhang, Xiuli,Xue, Xingya,Guo, Zhimou,&Liang, Xinmiao.(2011).Protein separation on a polar-copolymerized C-8 stationary phase.analytical and bioanalytical chemistry,399(10),3415-3421. |
| MLA | Dong, Jun,et al."Protein separation on a polar-copolymerized C-8 stationary phase".analytical and bioanalytical chemistry 399.10(2011):3415-3421. |
入库方式： OAI收割
来源：大连化学物理研究所
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