Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry
文献类型：期刊论文
| 作者 | Dong Jun1; Cai Xiao-ming1; Zou Li-juan2; Chen Cheng1; Xue Xing-ya1; Zhang Xiu-li1; Liang Xin-miao1 |
| 刊名 | chemical research in chinese universities
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 27期号:5页码:750-755 |
| 关键词 | Metabonomics Sample preparation Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC/Q-TOF MS) Lung cancer Biomarker |
| 英文摘要 | centrifugal ultrafiltration after methanol extraction of whole plasma was used as an optimal condition for the preparation of blood plasma before metabonomic studies. the plasma samples from 102 lung cancer patients and 34 healthy volunteers were prepared with this approach. with ultra-performance liquid chromatography(uplc) coupled with quadrupole time-of-flight mass spectrometry(q-tof ms) analysis, the samples were investigated in order to find potential disease biomarkers. after data acquisition, orthogonal signal correction partial least squares models were built to differentiate the healthy volunteers from lung cancer patients and to identify metabolites that showed significantly different expression between the two groups. several metabolite ions were identified as potential biomarkers according to the variable importance in the project(vip) value in both ion modes. five lysophosphatidylcholines were further identified as specifically lysopc 16:0, isomer of lysopc 16:0, lysopc 18:0, lysopc 18:1 and lysopc 18:2. these results suggest that uplc coupled with q-tof ms is an effective technique for the analysis of plasma metabolites in metabonomic studies. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | early-diagnosis ; structural determination ; sample preparation ; human plasma ; uplc-ms ; metabonomics ; serum ; precipitation ; metabolites ; statistics |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296120800009 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142320]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong Jun,Cai Xiao-ming,Zou Li-juan,et al. Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry[J]. chemical research in chinese universities,2011,27(5):750-755. |
| APA | Dong Jun.,Cai Xiao-ming.,Zou Li-juan.,Chen Cheng.,Xue Xing-ya.,...&Liang Xin-miao.(2011).Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry.chemical research in chinese universities,27(5),750-755. |
| MLA | Dong Jun,et al."Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry".chemical research in chinese universities 27.5(2011):750-755. |
入库方式： OAI收割
来源：大连化学物理研究所
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