In-column "click" preparation of hydrophobic organic monolithic stationary phases for protein separation
文献类型：期刊论文
| 作者 | Sun, Xiangli1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2 |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 399期号:10页码:3407-3413 |
| 关键词 | Click chemistry High-performance liquid chromatography Polymer monolithic column Protein separation |
| 英文摘要 | two types of macroporous organic polymer monoliths based on glycidyl methacrylate (gma), 4-vinylbenzyl chloride (vbc) and divinylbenzene (dvb) were prepared inside stainless-steel tubes. azide functionalities were firstly introduced on the surfaces of poly(gma-co-dvb) and poly(vbc-co-dvb) monoliths to provide reactive sites for click chemistry. with the application of copper(i)-catalyzed (3 + 2) azide-alkyne cycloaddition, an in-column click-modification approach for covalent attachment of long alkyl chains onto polymer monoliths was developed. the column morphology and surface chemistry of the fabricated monolithic columns were characterized by the scanning electron microscopy, mercury intrusion porosimeter, fourier transform infrared spectroscopy, and elemental analyses, respectively. the chromatographic performances of the "clicked" stationary phases were demonstrated with the high separation efficiency for a variety of proteins within 4 min. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; rigid polymer monoliths ; capillary electrochromatography ; efficient strategy ; terminal alkynes ; chemistry ; media ; supports ; facile ; azides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288398700010 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142323]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Xiangli,He, Xiwen,Chen, Langxing,et al. In-column "click" preparation of hydrophobic organic monolithic stationary phases for protein separation[J]. analytical and bioanalytical chemistry,2011,399(10):3407-3413. |
| APA | Sun, Xiangli,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2011).In-column "click" preparation of hydrophobic organic monolithic stationary phases for protein separation.analytical and bioanalytical chemistry,399(10),3407-3413. |
| MLA | Sun, Xiangli,et al."In-column "click" preparation of hydrophobic organic monolithic stationary phases for protein separation".analytical and bioanalytical chemistry 399.10(2011):3407-3413. |
入库方式： OAI收割
来源：大连化学物理研究所
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