Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis
文献类型：期刊论文
| 作者 | Cai, Xiaoming; Wang, Chaoran; Xu, Junyan; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2011-03-15 |
| 卷号 | 879期号:9-10页码:657-661 |
| 关键词 | Endogenous peptides Matrix solid-phase dispersion extraction NanoLC-MS/MS Peptide extraction Porcine hypothalamus |
| 英文摘要 | in this study, we investigated a novel application of matrix solid-phase dispersion (mspd) methodology for the extraction of endogenous peptides from porcine hypothalamus tissue samples. several experimental factors of the mspd procedure were examined. finally, silica-based octadecyl was chosen as dispersing material and blended with 0.25 g porcine hypothalamus at a ratio of 5, and 10 ml of 60% acetonitrile with 0.2% formic acid in water was chosen as the extraction and elution solvent. this mspd extraction method was compared to the classic acid extraction method. more peaks were observed in the mspd extracts (74 +/- 5) by maldi-tof ms than in acid extracts (34 +/- 5). moreover, 14 potential endogenous peptides were identified in the mspd extracts after nanolc-ms/ms analysis, while only 2 endogenous peptides in the acid extracts. these results indicated that mspd could be employed as a simple and efficient method for the extraction of endogenous peptides from tissues. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; liquid-chromatography ; neuropeptidomics ; optimization ; peptidomics ; discovery ; plasma ; ms/ms ; eggs |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288723500016 |
| 公开日期 | 2015-11-17 |
| 源URL | [http://159.226.238.44/handle/321008/142336]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Xiaoming,Wang, Chaoran,Xu, Junyan,et al. Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2011,879(9-10):657-661. |
| APA | Cai, Xiaoming,Wang, Chaoran,Xu, Junyan,Xue, Xingya,Zhang, Xiuli,&Liang, Xinmiao.(2011).Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis.journal of chromatography b-analytical technologies in the biomedical and life sciences,879(9-10),657-661. |
| MLA | Cai, Xiaoming,et al."Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis".journal of chromatography b-analytical technologies in the biomedical and life sciences 879.9-10(2011):657-661. |
入库方式： OAI收割
来源：大连化学物理研究所
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